Screening and cocktail optimization using experimental mixture design : enzymatic saccharification as a biological pretreatment strategy

• Biofuels contribute environment-friendly, renewable energy, minimizing dependence on fossil energy. The pretreatment of biomass is a practical step that accelerates and facilitates the hydrolysis of complex materials. This work aimed to screen, select, and study cocktail formulations for biomass hydrolysis, where the enzymes were provided both from a commercial source (Trichoderma reesei, Novozymes®) and through the cultivation of Aspergillus brasiliensis and Aspergillus tamarii Kita. Experimental mixture designs were used to optimize the enzymatic conversion of substrates into simple sugars. A crude extract rich in amylase (AAB) had a significant favorable influence on cornmeal hydrolysis by maximizing the yield of reducing sugars (RS) (173 μmol mL–1). Celluclast™, rich in cellulase, significantly affected the hydrolysis of banana peel, maximizing the RS yield (175 μmol mL–1). Variable degrees of enzyme synergism were evident from statistical analysis of the biomass hydrolysis.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)

Nyckelord

banana peel

biomass

cornmeal

enzymatic hydrolysis

experimental mixture design

sorghum grain bran

Aspergillus

Chemical industry

Enzymes

Saccharification

Sugars

Biological pre-treatment

Biomass hydrolysis

Commercial sources

Environment friendly

Enzymatic conversions

Enzymatic saccharification

Experimental mixture designs

Renewable energies

Mixtures

Resource Recovery

Resursåtervinning

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)