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Boosting enzymatic ...
Boosting enzymatic degradation of cellulose using a fungal expansin : Structural insight into the pretreatment mechanism
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- Ding, Sunjia (author)
- State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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- Liu, Xiaoqing (author)
- Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
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- Hakulinen, Nina (author)
- Department of Chemistry, University of Eastern Finland, Joensuu 80130, Finland
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- Taherzadeh, Mohammad J, 1965- (author)
- Högskolan i Borås,Akademin för textil, teknik och ekonomi,Swedish Centre for Resource Recovery
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- Wang, Yaru (author)
- State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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- Wang, Yuan (author)
- State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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- Qin, Xing (author)
- State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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- Wang, Xiaolu (author)
- State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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- Yao, Bin (author)
- State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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- Luo, Huiying (author)
- State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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- Tu, Tao (author)
- State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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(creator_code:org_t)
- Elsevier, 2022
- 2022
- English.
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In: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 358
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The recalcitrance of cellulosic biomass greatly hinders its enzymatic degradation. Expansins induce cell wall loosening and promote efficient cellulose utilization; however, the molecular mechanism underlying their action is not well understood. In this study, TlEXLX1, a fungal expansin from Talaromyces leycettanus JCM12802, was characterized in terms of phylogeny, synergy, structure, and mechanism of action. TlEXLX1 displayed varying degrees of synergism with commercial cellulase in the pretreatment of corn straw and filter paper. TlEXLX1 binds to cellulose via domain 2, mediated by CH–π interactions with residues Tyr291, Trp292, and Tyr327. Residues Asp237, Glu238, and Asp248 in domain 1 form hydrogen bonds with glucose units and break the inherent hydrogen bonding within the cellulose matrix. This study identified the expansin amino acid residues crucial for cellulose binding, and elucidated the structure and function of expansins in cell wall networks; this has potential applications in biomass utilization.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
Keyword
- Cellulose
- Expansin
- Pretreatment
- Cellulase
- Biomass degradation
- Crystallinity
- Resource Recovery
- Resursåtervinning
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Ding, Sunjia
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Liu, Xiaoqing
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Hakulinen, Nina
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Taherzadeh, Moha ...
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Wang, Yaru
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Wang, Yuan
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show more...
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Qin, Xing
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Wang, Xiaolu
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Yao, Bin
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Luo, Huiying
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Tu, Tao
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show less...
- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
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and Other Industrial ...
- Articles in the publication
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Bioresource Tech ...
- By the university
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University of Borås