Sökning: id:"swepub:oai:DiVA.org:hh-17454" >
Myogenic reprogramm...
Myogenic reprogramming of bone marrow derived cells in a W⁴¹Dmd(mdx) deficient mouse model
-
- Walsh, Stuart (författare)
- Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden
-
- Nygren, Jens (författare)
- Lund University,Lunds universitet,Högskolan i Halmstad,Centrum för forskning om välfärd, hälsa och idrott (CVHI),Hälsoinnovationer för barns psykiska hälsa,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
-
- Pontén, Annica (författare)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden
-
visa fler...
-
- Jovinge, Stefan (författare)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden
-
visa färre...
-
(creator_code:org_t)
- 2011-11-28
- 2011
- Engelska.
-
Ingår i: PLOS ONE. - San Francisco : Public Library of Science. - 1932-6203. ; 6:11, s. e27500-
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://hh.diva-port... (primary) (Raw object)
-
https://journals.plo...
-
https://portal.resea... (primary) (free)
-
http://www.ncbi.nlm....
-
http://dx.doi.org/10...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://lup.lub.lu.s...
-
visa färre...
Abstract
Ämnesord
Stäng
- Lack of expression of dystrophin leads to degeneration of muscle fibers and infiltration of connective and adipose tissue. Cell transplantation therapy has been proposed as a treatment for intractable muscle degenerative disorders. Several reports have demonstrated the ability of bone-marrow derived cells (BMDC) to contribute to non-haematopoietic tissues including epithelium, heart, liver, skeletal muscle and brain following transplantation by means of fusion and reprogramming. A key issue is the extent to which fusion and reprogramming can occur in vivo, particularly under conditions of myogenic deterioration.To investigate the therapeutic potential of bone marrow transplantation in monogenetic myopathy, green fluorescent protein-positive (GFP+) bone marrow cells were transplanted into non-irradiated c-kit receptor-deficient (W⁴¹) mdx mice. This model allows BMDC reconstitution in the absence of irradiation induced myeloablation. We provide the first report of BMDC fusion in a W⁴¹Dmd(mdx) deficient mouse model.In the absence of irradiation induced injury, few GFP+ cardiomyocytes and muscle fibres were detected 24 weeks post BMT. It was expected that the frequency of fusion in the hearts of W⁴¹Dmd(mdx) mice would be similar to frequencies observed in infarcted mice. Although, it is clear from this study that individual cardiomyocytes with monogenetic deficiencies can be rescued by fusion, it is as clear that in the absence of irradiation, the formation of stable and reprogrammed fusion hybrids occurs, with the current techniques, at very low levels in non-irradiated recipients.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- dystrophin
- green fluorescent protein
- stem cell factor receptor
- animal cell
- animal experiment
- animal model
- animal tissue
- article
- bone marrow cell
- bone marrow transplantation
- cell fusion
- cellular distribution
- controlled study
- Duchenne muscular dystrophy
- gene frequency
- heart infarction
- heart muscle cell
- hematopoietic stem cell transplantation
- irradiation
- mouse
- muscle cell
- nonhuman
- nuclear reprogramming
- protein expression
- protein localization
- soleus muscle
- tibialis anterior muscle
- tissue distribution
- X chromosome linked muscular dystrophic mouse
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
PLOS ONE
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas