Gene expression of angiogenic factors in muscle tissue during age-related development of hypertension in spontaneously hypertensive rats

• Essential hypertension has been associated with capillary rarefaction but little is known about the cellular mechanisms underlying this process. We examined the gene expression of angiogenic factors during age-related development of hypertension in spontaneously hypertensive rats (SHR). Wild-type Wistar Kyoto (WKY) rats served as controls. White gastrocnemius muscle was obtained and blood pressure was monitored at 5, 10 and 15 weeks of age. In the SHR group, systolic blood pressure increased from 5 to 10 and 15 weeks of age and the levels were higher than in the WKY group at 10 and 15 weeks (~70%; P<0.05). The mRNA content for MMP-2 was overall lower (P<0.05) in SHR compared to WKY. VEGFmRNA increased (p<0.05) from 5 to 10 weeks in SHR and there was a general increase (P<0.05) in the VEGF receptor flt-1. There was a trend for a lower content of eNOS and CYP 2C11 in the SHR than in WKY group. There were no alterations in the mRNA content of KDR, AMP 5'- nucleotidase, or Cytochrome P450 4A. The results show that the age-related development of hypertension from 5 to 15 weeks in SHR rats is not associated with major changes in mRNA content of the herein included angiogenic factors.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Physiology (hsv//eng)

Nyckelord

angiogenesis hypertension

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