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Targeted inhibition...
Targeted inhibition of ATP5B gene prevents bone erosion in collagen-induced arthritis by inhibiting osteoclastogenesis.
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- Xu, Yanting (författare)
- Southern Medical University, Guangzhou, China
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- Tan, Huijing (författare)
- Southern Medical University, Guangzhou, China
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- Liu, Kaifei (författare)
- Southern Medical University, Guangzhou, China
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- Wen, Cailing (författare)
- Southern Medical University, Guangzhou, China
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- Pang, Caixia (författare)
- Southern Medical University, Guangzhou, China
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- Liu, Haiqian (författare)
- Southern Medical University, Guangzhou, China
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- Xu, Rui (författare)
- Southern Medical University, Guangzhou, China
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- Li, Qixing (författare)
- Southern Medical University, Guangzhou, China
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- He, Chonghua (författare)
- Southern Medical University, Guangzhou, China
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- Nandakumar, Kutty Selva, 1965- (författare)
- Southern Medical University, Guangzhou, China
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- Zhou, Chun (författare)
- Southern Medical University, Guangzhou, China
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(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- Engelska.
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Ingår i: Pharmacological Research. - : Elsevier BV. - 1043-6618 .- 1096-1186. ; 165
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Bone resorption by osteoclasts is an energy consuming activity, which depends on mitochondrial ATP. ATP5B, a mitochondrial ATP synthase beta subunit, is a catalytic core involved in producing ATP. Here, we investigated the contribution of ATP5B in osteoclast differentiation and joint destruction. ATP5B (LV-ATP5B) targeting or non-targeting (LV-NC) siRNA containing lentivirus particles were transduced into bone marrow macrophage derived osteoclasts or locally administered to arthritic mouse joints. Inhibition of ATP5B reduced the expression of osteoclast related genes and proteins, suppressed bone resorption by significantly impairing F-actin formation and decreased the levels of adhesion-associated proteins. In addition, ATP5B deficiency caused osteoclast mitochondrial dysfunction and, impaired the secretion of vacuole protons and MMP9. Importantly, inhibition of ATP5B expression, protected arthritis mice from joint destructions although serum levels of inflammatory mediators (TNF-α, IL-1β) and IgG2α antibodies were unaffected. These results demonstrate an essential function of ATP5B in osteoclast differentiation and bone resorption, and suggest it as a potential therapeutic target for protecting bones in RA.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Nyckelord
- ATP5B
- CIA
- Mitochondrial metabolism
- Osteoclasts
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Xu, Yanting
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Tan, Huijing
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Liu, Kaifei
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Wen, Cailing
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Pang, Caixia
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Liu, Haiqian
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visa fler...
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Xu, Rui
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Li, Qixing
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He, Chonghua
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Nandakumar, Kutt ...
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Zhou, Chun
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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