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Tatanan A from the ...
Tatanan A from the Acorus calamus L. root inhibited dengue virus proliferation and infections
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- Yao, Xingang (författare)
- School of Pharmaceutical Sciences, Southern Medical University, Guangzhou
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- Ling, Yun (författare)
- Jiujiang University, Jiujiang, China
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- Guo, Songxin (författare)
- School of Pharmaceutical Sciences, Southern Medical University, Guangzhou
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- Wu, Wenyu (författare)
- Sun Yat-sen University, Guangzhou, China
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- He, Shijun (författare)
- School of Pharmaceutical Sciences, Southern Medical University, Guangzhou
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- Zhang, Qing (författare)
- Jiujiang University, Jiujiang, China
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- Zou, Min (författare)
- School of Pharmaceutical Sciences, Southern Medical University, Guangzhou
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- Nandakumar, Kutty Selva, 1965- (författare)
- School of Pharmaceutical Sciences, Southern Medical University, Guangzhou
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- Chen, Xiaoguang (författare)
- Southern Medical University, Guangzhou, China
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- Liu, Shuwen (författare)
- School of Pharmaceutical Sciences, Southern Medical University, Guangzhou
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(creator_code:org_t)
- München : Elsevier, 2018
- 2018
- Engelska.
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Ingår i: Phytomedicine. - München : Elsevier. - 0944-7113 .- 1618-095X. ; 42, s. 258-267
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- BACKGROUND: Acorus calamus l. (Acoraceae) is a well-known traditional Chinese medicinal plant, whose root are historically mainly used to treat neurodegenerative diseases, and for cholera treatment. This datum strongly indicates the antimicrobial activity of A. calamus.PURPOSE: Our goal is to find the active constituents of A. calamus to treat dengue virus (DENV) infections, and to study the effects and mechanisms of these active substances.METHODS: The root of A. calamus was extracted by ethanol. Mosquito larva C6/36 cells were used for DENV2 replication and transfection host. Mouse kidney fibroblast cells (BHK-21) were used as a host cell to study the infection ability of the virus. DENV2-induced cytopathic effect (CPE) and plaque assay were used to evaluate the inhibitory effect of A. calamus extracts on DENV2 infectivity inhibition. The levels of E and NS1 protein expression were measured by real-time PCR and western blot assays.RESULTS: 12 compounds were isolated from ethanol extract of A. calamus root, tatanan A showed the best anti-DENV ability among these 12 compounds, which significantly alleviated DENV2-induced CPE and cytotoxicity effects, with an EC50 of 3.9 µM. In addition, RNA replication assay further confirmed the antivirus ability of tatanan A. Time-addition assay showed that tatanan A affected the early stage of viral RNA replication, which in turn inhibited mRNA and protein levels of DENV2.CONCLUSIONS: These results demonstrated the anti-DENV2 effect of tatanan A, in inhibiting DENV2 RNA replication and infections. In summary, tatanan A was found to be a novel natural DENV inhibitor and a potential candidate for the treatment of DENV infectious disease.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Nyckelord
- Acorus calamus l
- DENV2 infection
- Dengue
- Tatanan A
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Yao, Xingang
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Ling, Yun
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Guo, Songxin
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Wu, Wenyu
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He, Shijun
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Zhang, Qing
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visa fler...
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Zou, Min
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Nandakumar, Kutt ...
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Chen, Xiaoguang
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Liu, Shuwen
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Farmaceutiska ve ...
- Artiklar i publikationen
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Phytomedicine
- Av lärosätet
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Högskolan i Halmstad