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Evaluation of the p...
Evaluation of the percentage of peripheral T cells with two different T cell receptor alpha-chains and of their potential role in autoimmunity
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- Corthay, Alexandre (författare)
- University of Oslo, National Hospital, Oslo, Norway; Lund University, Lund, Sweden
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- Nandakumar, Kutty Selva, 1965- (författare)
- Lund University, Lund, Sweden
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- Holmdahl, Rikard (författare)
- Lund University, Lund, Sweden
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(creator_code:org_t)
- Rockville, MD : American Association of Immunologists, 2001
- 2001
- Engelska.
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Ingår i: Journal of Autoimmunity. - Rockville, MD : American Association of Immunologists. - 0896-8411 .- 1095-9157. ; 16:4, s. 423-429
- Relaterad länk:
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https://urn.kb.se/re...
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visa fler...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Approximately 25% of mature T cells possess two distinct cytoplasmic T cell receptor (TCR) alpha-chains, due to productive gene rearrangements of both alleles. Expression of two different alpha-chains at the cell surface is a potential risk factor for development of autoimmunity. However, it has been difficult to determine the frequency of peripheral T cells with two different alpha-chains at the surface. Our new approach is based on comparing by flow cytometry the percentage of cells that express a given Valpha-chain between wild-type mice and mice that are hemizygous for a disrupted Tcra locus (Tcra+/-) and consequently unable to express two rearranged Tcra genes. We consistently found that approximately 8% of total peripheral T cells express two surface alpha-chains. The importance of dual alpha-T cells in autoimmunity was examined in a mouse model for rheumatoid arthritis, namely collagen-induced arthritis (CIA). No significant difference was observed between Tcra+/- mice and wild-type littermates, considering arthritis incidence, day of disease onset, and maximum arthritic score. We therefore conclude that there is incomplete phenotypic allelic exclusion in TCRalpha, and that the presence of a significant number of potentially multireactive T cells does not increase the susceptibility to develop autoimmune arthritis. © 2001 Academic Press.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Allelic exclusion
- Autoimmunity
- Collagen-induced arthritis
- Rheumatoid arthritis
- TCR α
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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