Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells

• Antibodies against type II collagen (anti-CII) are arthritogenic and have a crucial role in the initiation of collagen-induced arthritis. Here, we have determined the dependence of T and B cells in collagen-antibody-induced arthritis (CAIA) during different phases of arthritis. Mice deficient for B and/or T cells were susceptible to the CAIA, showing that the antibodies induce arthritis even in the absence of an adaptive immune system. To determine whether CII-reactive T cells could have a role in enhancing arthritis development at the effector level of arthritis pathogenesis, we established a T cell line reactive with CII. This T cell line was oligoclonal and responded to different post-translational forms of the major CII epitope at position 260-270 bound to the Aq class II molecule. Importantly, it cross-reacted with the mouse peptide although it is bound with lower affinity to the Aq molecule than the corresponding rat peptide. The T cell line could not induce clinical arthritis per se in Aq-expressing mice even if these mice expressed the major heterologous CII epitope in cartilage, as in the transgenic MMC (mutated mouse collagen) mouse. However, a combined treatment with anti-CII monoclonal antibodies and CII-reactive T cells enhanced the progression of severe arthritis.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)

Nyckelord

Adoptive Transfer

Animals

Antibodies

Monoclonal/immunology/*toxicity

Antibody Specificity

Arthritis

Experimental/*immunology

B-Lymphocytes/immunology

Collagen Type II/chemistry/*immunology

Crosses

Genetic

Disease Progression

Epitopes

T-Lymphocyte/*immunology

Female

Glycosylation

Histocompatibility Antigens Class II/immunology

Immunity

Cellular

Immunization

Passive

Immunologic Deficiency Syndromes/immunology

Male

Mice

Mice

Inbred C3H/immunology

Mice

Inbred C57BL

Mice

Inbred DBA

Mice

Knockout

Protein Processing

Post-Translational

Rats

Receptors

Antigen

T-Cell

alpha-beta/deficiency

T-Lymphocyte Subsets/*immunology

T cells

monoclonal antibodies

collagen type II

arthritis

B cells

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)