Sökning: id:"swepub:oai:DiVA.org:hh-48892" >
Macrophage-derived ...
Macrophage-derived reactive oxygen species protects against autoimmune priming with a defined polymeric adjuvant
-
- Shakya, Akhilesh Kumar (författare)
- Karolinska Institute, Stockholm, Sweden; Texas Tech University, Lubbock, TX, United States,Karolinska Institute, Stockholm, Sweden; Texas Tech University, Lubbock, TX, USA
-
- Kumar, Ashok (författare)
- Indian Institute of Technology Kanpur, Kanpur, India
-
- Holmdahl, Rikard (författare)
- Karolinska Institutet,Karolinska Institute, Stockholm, Sweden; Southern Medical University, Guangzhou, China
-
visa fler...
-
- Nandakumar, Kutty Selva, 1965- (författare)
- Karolinska Institutet,Karolinska Institute, Stockholm, Sweden; University of Arkansas for Medical Sciences, Little Rock, AR, United States,Karolinska Institute, Stockholm, Sweden; University of Arkansas for Medical Sciences, Little Rock, AR, USA
-
visa färre...
-
(creator_code:org_t)
- 2015-11-24
- 2016
- Engelska.
-
Ingår i: Immunology. - Chichester : Wiley-Blackwell. - 0019-2805 .- 1365-2567. ; 147:1, s. 125-132
- Relaterad länk:
-
https://onlinelibrar...
-
visa fler...
-
https://hh.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Understanding the nature of adjuvants and the immune priming events in autoimmune diseases, such as rheumatoid arthritis, is a key challenge to identify their aetiology. Adjuvants are, however, complex structures with inflammatory and immune priming properties. Synthetic polymers provide a possibility to separate these functions and allow studies of the priming mechanisms in vivo. A well-balanced polymer, poly-N-isopropyl acrylamide (PNiPAAm) mixed with collagen type II (CII) induced relatively stronger autoimmunity and arthritis compared with more hydrophilic (polyacrylamide) or hydrophobic (poly-N-isopropylacrylamide-co-poly-N-tertbutylacrylamide and poly-N-tertbutylacrylamide) polymers. Clearly, all the synthesized polymers except the more hydrophobic poly-N-tertbutylacrylamide induced arthritis, especially in Ncf1-deficient mice, which are deficient in reactive oxygen species (ROS) production. We identified macrophages as the major infiltrating cells present at PNiPAAm-CII injection sites and demonstrate that ROS produced by the macrophages attenuated the immune response and the development of arthritis. Our results reveal that thermo-responsive polymers with high immune priming capacity could trigger an autoimmune response to CII and the subsequent arthritis development, in particular in the absence of NOX2 derived ROS. Importantly, ROS from macrophages protected against the autoimmune priming, demonstrating a critical regulatory role of macrophages in immune priming events. © 2016 John Wiley & Sons Ltd.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
Nyckelord
- Adjuvant
- Arthritis
- Collagen type II
- Macrophages
- Poly-N-isopropylacrylamide
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas