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Spatiotemporal extr...
Spatiotemporal extracellular matrix modeling for in situ cell niche studies
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- Olesen, Kim (författare)
- Uppsala universitet,Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden ; Polymer Chemistry, Department of Chemistry – Ångström Laboratory, Uppsala University, Sweden,Infektionsbiologi, Infection Biology,Polymerkemi,Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden; Univ Skövde, Sch Biosci, Skövde, Sweden,Karolinska Inst, Sweden; Univ Skovde, Sweden; Uppsala Univ, Sweden
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- Rodin, Sergey (författare)
- Uppsala universitet,Thoraxkirurgi,Uppsala Univ, Sweden
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- Mak, Wing Cheung (författare)
- Linköpings universitet,Sensor- och aktuatorsystem,Tekniska fakulteten
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- Felldin, Ulrika (författare)
- Uppsala universitet,Thoraxkirurgi,Uppsala Univ, Sweden
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- Österholm, Cecilia (författare)
- Karolinska Institutet,Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden.
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- Tilevik, Andreas (författare)
- Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,Infektionsbiologi, Infection Biology,Univ Skövde, Sch Biosci, Skövde, Sweden.
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- Grinnemo, Karl-Henrik (författare)
- Uppsala universitet,Thoraxkirurgi,Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden,Karolinska Inst, Sweden; Uppsala Univ, Sweden
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(creator_code:org_t)
- 2021-08-31
- 2021
- Engelska.
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Ingår i: Stem Cells. - : John Wiley & Sons. - 1066-5099 .- 1549-4918. ; 39:12, s. 1751-1765
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Abstract
Ämnesord
Stäng
- Extracellular matrix (ECM) components govern a range of cell functions such as migration, proliferation, maintenance of stemness and differentiation. Cell niches that harbor stem-/progenitor cells, with matching ECM, have been shown in a range of organs, although their presence in the heart is still under debate. Determining niches depends on a range of in vitro and in vivo models and techniques, where animal models are powerful tools for studying cell-ECM dynamics, however, they are costly and time-consuming to use. In vitro models based on recombinant ECM proteins lack the complexity of the in vivo ECM. To address these issues, we present the Spatiotemporal Extracellular Matrix Model (StEMM) for studies of cell-ECM dynamics, such as cell niches. This model combines gentle decellularization and sectioning of cardiac tissue, allowing retention of a complex ECM, with recellularization and subsequent image processing using image stitching, segmentation, automatic binning and generation of cluster maps. We have thereby developed an in situ representation of the cardiac ECM that is useful for assessment of repopulation dynamics and to study the effect of local ECM composition on phenotype preservation of reseeded mesenchymal progenitor cells. This model provides a platform for studies of organ-specific cell-ECM dynamics and identification of potential cell niches. © AlphaMed Press 2021 SIGNIFICANCE STATEMENT: Stem cells reside in adult organs within specific microenvironments called cell niches. The heart is a complex organ and so far, the presence and localization of stem-/progenitor cell niches are subject to constant debate. To address these issues, the authors have developed the Spatiotemporal Extracellular Matrix Model (StEMM), which combines a modified protocol for decellularization, with cryo-sectioning, recellularization, and subsequent image processing including automatic binning and generation of cluster maps. StEMM was developed within a cardiac context and validated using syngeneic mesenchymal progenitor cells. However, this model is not restricted with regard to species or organs.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- cardiac
- mesenchymal stem cells
- multipotential differentiation
- pericytes
- progenitor cells
- scaffold attachment region
- stem cell-microenvironment interactions
- technology
- Infection Biology
- Infektionsbiologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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