Nitric oxide-dependent in vitro secretion of amylase from innervated or chronically denervated parotid glands of the rat in response to isoprenaline and vasoactive intestinal peptide

• The basal in vitro release of amylase was similar from rat parotid lobules of innervated and chronically denervated glands and was unaffected by the inhibitors used in this study. The secretion of amylase induced by isoprenaline or vasoactive intestinal peptide (VIP) was reduced by one-third to one-half from the lobules of the innervated glands and even more so from the lobules of the denervated glands by ODQ, an inhibitor of soluble guanyl cyclase which is activated by nitric oxide (NO) and catalyses the cGMP production. The use of Nω-propyl-L-arginine (N-PLA) revealed that the evoked secretion of amylase in the denervated glands depended on the activity of neuronal type NO synthase to synthesize NO. Since the denervated gland is virtually devoid of NO synthase-containing nerve fibres, the neuronal type NO synthase was most probably of a non-neuronal source. NO-dependent amylase secretion was agonist related, since amylase secretion evoked by bethanechol and neuropeptide Y was not reduced by ODQ or N-PLA. Hence, under physiological conditions, activation of β-adrenoceptors (sympathetic activity) and VIP receptors (parasympathetic activity) is likely to cause secretion of parotid amylase partly through a NO/cGMP-dependent intracellular pathway involving the activity of neuronal type NO synthase, possibly of acinar origin.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Physiology (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Odontologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Dentistry (hsv//eng)

Nyckelord

1h 1

2

4 oxadiazolo[4

3 a]quinoxalin 1 one

amylase

arginine derivative

beta adrenergic receptor

bethanechol

cyclic GMP

guanylate cyclase inhibitor

isoprenaline

neuropeptide Y

nitric oxide

nitric oxide synthase

vasoactive intestinal polypeptide

vasoactive intestinal polypeptide receptor

amylase release

animal tissue

article

catalysis

cholinergic activity

controlled study

denervation

enzyme activity

female

in vitro study

innervation

nerve fiber

nonhuman

parotid gland

rat

sympathetic tone

Adrenergic beta-Agonists

Amylases

Animals

Arginine

Autonomic Denervation

Enzyme Inhibitors

Gastrointestinal Agents

Guanylate Cyclase

Isoproterenol

Oxadiazoles

Parasympathomimetics

Quinoxalines

Rats

Rats

Sprague-Dawley

Vasoactive Intestinal Peptide

Adrenergic beta-Agonists

pharmacology

Amylases

secretion

Animals

Arginine

analogs & derivatives

Autonomic Denervation

Bethanechol

Enzyme Inhibitors

Female

Gastrointestinal Agents

Guanylate Cyclase

antagonists & inhibitors

Isoproterenol

Neuropeptide Y

Nitric Oxide

metabolism

Nitric Oxide Synthase

Oxadiazoles

Parasympathomimetics

Parotid Gland

drug effects

innervation

Quinoxalines

Rats

Sprague-Dawley

Vasoactive Intestinal Peptide

Publikations- och innehållstyp

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art (ämneskategori)