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A Role for the 2' O...
A Role for the 2' OH of peptidyl-tRNA substrate in peptide release on the ribosome revealed through RF-mediated rescue
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- Shaw, Jeffrey J. (författare)
- Johns Hopkins University School of Medicine, Baltimore
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- Trobro, Stefan, 1972- (författare)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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- He, Shan L. (författare)
- Johns Hopkins University School of Medicine, Baltimore
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- Åqvist, Johan (författare)
- Uppsala universitet,Beräknings- och systembiologi
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- Green, Rachel (författare)
- Johns Hopkins University School of Medicine, Baltimore
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(creator_code:org_t)
- 2012
- 2012
- Engelska.
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Ingår i: Chemistry and Biology. - 1074-5521 .- 1879-1301. ; 19:8, s. 983-993
- Relaterad länk:
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https://doi.org/10.1...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The 2' OH of the peptidyl-tRNA substrate is thought to be important for catalysis of both peptide bond formation and peptide release in the ribosomal active site. The release reaction also specifically depends on a release factor protein (RF) to hydrolyze the ester linkage of the peptidyl-tRNA upon recognition of stop codons in the A site. Here, we demonstrate that certain amino acid substitutions (in particular those containing hydroxyl or thiol groups) in the conserved GGQ glutamine of release factor RF1 can rescue defects in the release reaction associated with peptidyl-tRNA substrates lacking a 2' OH. We explored this rescue effect through biochemical and computational approaches that support a model where the 2' OH of the P-site substrate is critical for orienting the nucleophile in a hydrogen-bonding network productive for catalysis.
Ämnesord
- NATURAL SCIENCES -- Chemical Sciences -- Theoretical Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi -- Teoretisk kemi (hsv//swe)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologiska vetenskaper (hsv//swe)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)