Sökning: id:"swepub:oai:DiVA.org:kth-102911" >
Validation of whole...
Validation of whole genome amplification for analysis of the p53 tumor suppressor gene in limited amounts of tumor samples
-
- Hasmats, Johanna (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,Science for Life Laboratory, School of Biotechnology, Division of Gene Technology, Royal Institute of Technology, Stockholm, Sweden
-
- Gréen, Henrik (författare)
- Linköpings universitet,KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,Avdelningen för läkemedelsforskning,Hälsouniversitetet,Science for Life Laboratory, School of Biotechnology, Division of Gene Technology, Royal Institute of Technology, Stockholm, Sweden
-
- Solnestam, Beata Werne (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,Science for Life Laboratory, School of Biotechnology, Division of Gene Technology, Royal Institute of Technology, Stockholm, Sweden
-
visa fler...
-
- Zajac, Pawel (författare)
- Laboratory for Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
-
- Huss, Mikael (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
- Orear, Cedric (författare)
- Genomics Unit, Institut Gustave Roussy, Villejuif, France
-
- Validire, Pierre (författare)
- Department of Pathology, Institut Mutualiste Montsouris, Paris, France
-
- Bjursell, Magnus (författare)
- AstraZeneca R&D, Mölndal, Sweden
-
- Lundeberg, Joakim (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,Science for Life Laboratory, School of Biotechnology, Division of Gene Technology, Royal Institute of Technology, Stockholm, Sweden
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- Engelska.
-
Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 425:2, s. 379-383
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://liu.diva-por... (primary) (Raw object)
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
https://urn.kb.se/re...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Personalized cancer treatment requires molecular characterization of individual tumor biopsies. These samples are frequently only available in limited quantities hampering genomic analysis. Several whole genome amplification (WGA) protocols have been developed with reported varying representation of genomic regions post amplification. In this study we investigate region dropout using a 929 polymerase based WGA approach. DNA from 123 lung cancers specimens and corresponding normal tissue were used and evaluated by Sanger sequencing of the p53 exons 5-8. To enable comparative analysis of this scarce material, WGA samples were compared with unamplified material using a pooling strategy of the 123 samples. In addition, a more detailed analysis of exon 7 amplicons were performed followed by extensive cloning and Sanger sequencing. Interestingly, by comparing data from the pooled samples to the individually sequenced exon 7, we demonstrate that mutations are more easily recovered from WGA pools and this was also supported by simulations of different sequencing coverage. Overall this data indicate a limited random loss of genomic regions supporting the use of whole genome amplification for genomic analysis.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Whole genome amplification
- TP53
- Mutations
- Validation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas