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Ultra-deep pyrosequ...
Ultra-deep pyrosequencing of hepatitis B virus quasispecies from nucleoside and nucleotide reverse-transcriptase inhibitor (NRTI)-treated patients and NRTI-naive patients
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Margeridon-Thermet, S (författare)
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Shulman, NS (författare)
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Ahmed, A (författare)
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Shahriar, R (författare)
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Liu, T (författare)
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Wang, C (författare)
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Holmes, SP (författare)
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- Babrzadeh, Farbod (författare)
- Stanford Genome Technology Centre, USA
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Gharizadeh, B (författare)
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Hanczaruk, B (författare)
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Simen, BB (författare)
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Egholm, M (författare)
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Shafer, RW (författare)
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(creator_code:org_t)
- University of Chicago Press, 2009
- 2009
- Engelska.
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Ingår i: Journal of Infectious Diseases. - : University of Chicago Press. - 0022-1899 .- 1537-6613. ; 199:9, s. 1275-1285
- Relaterad länk:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The dynamics of emerging nucleoside and nucleotide reverse-transcriptase inhibitor (NRTI) resistance in hepatitis B virus (HBV) are not well understood because standard dideoxynucleotide direct polymerase chain reaction (PCR) sequencing assays detect drug-resistance mutations only after they have become dominant. To obtain insight into NRTI resistance, we used a new sequencing technology to characterize the spectrum of low-prevalence NRTI-resistance mutations in HBV obtained from 20 plasma samples from 11 NRTI-treated patients and 17 plasma samples from 17 NRTI-naive patients, by using standard direct PCR sequencing and ultra-deep pyrosequencing (UDPS). UDPS detected drug-resistance mutations that were not detected by PCR in 10 samples from 5 NRTI-treated patients, including the lamivudine-resistance mutation V173L (in 5 samples), the entecavir-resistance mutations T184S (in 2 samples) and S202G (in 1 sample), the adefovir-resistance mutation N236T (in 1 sample), and the lamivudine and adefovir-resistance mutations V173L, L180M, A181T, and M204V (in 1 sample). G-to-A hypermutation mediated by the apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like family of cytidine deaminases was estimated to be present in 0.6% of reverse-transcriptase genes. Genotype A coinfection was detected by UDPS in each of 3 patients in whom genotype G virus was detected by direct PCR sequencing. UDPS detected low-prevalence HBV variants with NRTI-resistance mutations, G-to-A hypermutation, and low-level dual genotype infection with a sensitivity not previously possible.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
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Margeridon-Therm ...
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Shulman, NS
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Ahmed, A
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Shahriar, R
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Liu, T
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Wang, C
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visa fler...
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Holmes, SP
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Babrzadeh, Farbo ...
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Gharizadeh, B
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Hanczaruk, B
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Simen, BB
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Egholm, M
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Shafer, RW
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visa färre...
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