Oxidative cellulose degradation by cellobiose dehydrogenase from Phanerochaete chrysosporium : A model compound study

• Cellobiose dehydrogenase (CDH) is an extracellular enzyme produced by various wood-degrading fungi. It oxidizes cellobiose to cellobionolactone under reduction of molecular oxygen to hydrogen peroxide, and Fe3+ to Fe2+. These activated agents can thereafter form highly reactive hydroxyl radicals, which depolymerize wood polymers. In this work, cellulose depolymerization by CDH was studied using a model compound, methyl beta-D-glucopyranoside. The formation of glucose, arabinose, gluconic acid, erythrulose and formaldehyde were detected and a mechanism for the reaction is proposed. The biological importance of this enzyme-initiated reaction is discussed.

Nyckelord

cellobiose dehydrogenase

cellulose biodegradation

hydroxyl radical

methyl beta-D-glucopyranoside

white-rot fungus

sporotrichum-pulverulentum

quinone oxidoreductase

degrading enzyme

oxidase

delignification

purification

cellulases

mechanism

lignin

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