Controlling acoustic streaming in a multi-well microplate for improving live cell assays

• Acoustic streaming in a multi-well microplate is investigated using two different ultrasonic actuation frequency-schemes: Single-frequency and frequency-modulation. The streaming is tracked by the use of 1 μm fluorescent polymer beads and micro-particle image velocimetry. The suspension also contained human B cells for studying the acoustic trapping and aggregation performance simultaneously with the acoustic streaming. Our results show a significant difference in the acoustic streaming between the two ultrasonic actuation schemes. A rotational fluid flow speed decreased a factor of 30 when frequency-modulation was applied compared to single-frequency actuation without apparently interfering with the acoustic cell trapping function.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Acoustic streaming

Microplate

Mixing

Multi-well

Particle image velocimetry

Ultrasound

Cell trapping

Fluorescent polymers

Live cell

Micro particle image velocimetry

Microplates

Multi wells

Particle image velocimetries

Single-frequency

Flow visualization

Modulation

Ultrasonics

Velocimeters

Velocity measurement

Publikations- och innehållstyp

ref (ämneskategori)

kon (ämneskategori)