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Parallel dual-color...
Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements
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- Gosch, M. (författare)
- Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL),Karolinska Institutet
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- Blom, Hans (författare)
- Karolinska Institutet,Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL)
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- Anderegg, S. (författare)
- Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL),Karolinska Institutet
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- Korn, K. (författare)
- Karolinska Institutet,Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL)
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- Thyberg, P. (författare)
- Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL),Karolinska Institutet
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- Wells, M. (författare)
- Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL),Karolinska Institutet
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- Lasser, T. (författare)
- Karolinska Institutet,Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL)
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- Rigler, R. (författare)
- Karolinska Institutet,Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL)
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- Magnusson, A. (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Hård, Sverker, 1945 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- SPIE-Intl Soc Optical Eng, 2005
- 2005
- Engelska.
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Ingår i: Journal of Biomedical Optics. - : SPIE-Intl Soc Optical Eng. - 1083-3668 .- 1560-2281. ; 10:5
- Relaterad länk:
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633 nm) into four sub-beams with the help of two 2 X 2 fan-out diffractive optical elements (DOES), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2 x 2 fiber arrays.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
Nyckelord
- cross-correlation
- parallel
- fluorescence correlation spectroscopy
- single-molecule detection
- fan-out element
- gene-expression
- statistical accuracy
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Gosch, M.
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Blom, Hans
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Anderegg, S.
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Korn, K.
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Thyberg, P.
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Wells, M.
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visa fler...
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Lasser, T.
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Rigler, R.
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Magnusson, A.
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Hård, Sverker, 1 ...
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visa färre...
- Om ämnet
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Elektroteknik oc ...
- Artiklar i publikationen
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Journal of Biome ...
- Av lärosätet
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Kungliga Tekniska Högskolan
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Karolinska Institutet
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Chalmers tekniska högskola