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Analysis of protein...
Analysis of protein expression in cell microarrays : A tool for antibody-based proteomics
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- Andersson, Ann-Catrin (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Strömberg, Sara (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Backvall, Helena (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Kampf, Caroline (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Uhlén, Mathias (author)
- KTH,Proteomik
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- Wester, Kenneth (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Pontén, Fredrik (author)
- Uppsala universitet,Institutionen för genetik och patologi
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(creator_code:org_t)
- 2006
- 2006
- English.
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In: Journal of Histochemistry and Cytochemistry. - 0022-1554 .- 1551-5044. ; 54:12, s. 1413-1423
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
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- Tissue microarray (TMA) technology provides a possibility to explore protein expression patterns in a multitude of normal and disease tissues in a high-throughput setting. Although TMAs have been used for analysis of tissue samples, robust methods for studying in vitro cultured cell lines and cell aspirates in a TMA format have been lacking. We have adopted a technique to homogeneously distribute cells in an agarose gel matrix, creating an artificial tissue. This enables simultaneous profiling of protein expression in suspension- and adherent-grown cell samples assembled in a microarray. In addition, the present study provides an optimized strategy for the basic laboratory steps to efficiently produce TMAs. Presented modifications resulted in an improved quality of specimens and a higher section yield compared with standard TMA production protocols. Sections from the generated cell TMAs were tested for immunohistochemical staining properties using 20 well-characterized antibodies. Comparison of immunoreactivity in cultured dispersed cells and corresponding cells in tissue samples showed congruent results for all tested antibodies. We conclude that a modified TIVIA technique, including cell samples, provides a valuable tool for high-throughput analysis of protein expression, and that this technique can be used for global approaches to explore the human proteome.
Keyword
- immunohistochemistry
- cell line
- tissue microarray
- affinity proteomics
- antibody-based proteomics
- anticancer drug screen
- high-throughput
- tissue microarrays
- cultured-cells
- lines
- cancer
- immunohistochemistry
- quantification
- establishment
- technology
- MEDICINE
Publication and Content Type
- ref (subject category)
- art (subject category)
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