Protein A chromatography for antibody purification

• Staphylococcal protein A (SPA) is one of the first discovered immunoglobulin binding molecules and has been extensively studied during the past decades. Due to its affinity to immunoglobulins, SPA has found widespread use as a tool in the detection and purification of antibodies and the molecule has been further developed to one of the most employed affinity purification systems. Interestingly, a minimized SPA derivative has been constructed and a domain originating from SPA has been improved to withstand the harsh environment employed in industrial purifications. This review will focus on the development of different affinity molecules and matrices for usage in antibody purification.

Nyckelord

affinity chromatography

cleaning-in-place (CIP)

deamidation

protein A

protein engineering

stabilization

affinity-chromatography

monoclonal-antibodies

staphylococcus-aureus

synthetic ligand

immunoglobulin-g

phage display

binding

sorbents

deamidation

peptides

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