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Self-assembling sup...
Self-assembling supramolecular complexes by single-stranded extension from plasmid DNA
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- Svahn, Mathias G. (författare)
- Karolinska Institutet
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Hasan, Maroof (författare)
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Sigot, Valeria (författare)
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Valle-Delgado, Juan Jose (författare)
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- Rutland, Mark W. (författare)
- KTH,Ytkemi
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- Lundin, Karin E. (författare)
- Karolinska Institutet
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- Smith, C. I. Edvard (författare)
- Karolinska Institutet
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(creator_code:org_t)
- Mary Ann Liebert Inc, 2007
- 2007
- Engelska.
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Ingår i: Oligonucleotides. - : Mary Ann Liebert Inc. - 1545-4576 .- 1557-8526. ; 17:1, s. 80-94
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Self-assembling supramolecular complexes are of great interest for bottom-up research like nanotechnology. DNA is an inexpensive building block with sequence-specific self-assembling capabilities through Watson-Crick and/or Hoogsteen base pairing and could be used for applications in surface chemistry, material science, nanomechanics, nanoelectronics, nanorobotics, and of course in biology. The starting point is usually single-stranded DNA, which is rather easily accessible for base pairing and duplex formation. When long stretches of double-stranded DNA are desirable, serving either as genetic codes or electrical wires, bacterial expansion of plasmids is an inexpensive approach with scale-up properties. Here, we present a method for using double-stranded DNA of any sequence for generating simple structures, such as junctions and DNA lattices. It is known that supercoiled plasmids are strand-invaded by certain DNA analogs. Here we add to the complexity by using Selfassembling UNiversal (SUN) anchors formed by DNA analog oligonucleotides, synthesized with an extension, a sticky-end that can be used for further base pairing with single-stranded DNA. We show here how the same set of SUN anchors can be utilized for gene therapy, plasmid purification, junction for lattices, and plasmid dimerization through Watson-Crick base pairing. Using atomic force microscopy, it has been possible to characterize and quantify individual components of such supra-molecular complexes.
Nyckelord
- thymine-substituted polyamide
- nucleic-acids
- bioplex technology
- gene chemistry
- triplex dna
- duplex dna
- peptides
- pna
- oligonucleotides
- stability
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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