Search: id:"swepub:oai:DiVA.org:kth-173984" >
Genome-wide mapping...
Genome-wide mapping of promoter-anchored interactions with close to single-enhancer resolution
-
- Sahlén, Pelin (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
-
- Abdullayev, Ilgar (author)
- Karolinska Institutet
-
- Ramsköld, Daniel (author)
- Karolinska Institutet
-
show more...
-
- Matskova, Liudmila (author)
- Karolinska Institutet
-
- Rilakovic, Nemanja (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
-
- Lötstedt, Britta (author)
- KTH,Science for Life Laboratory, SciLifeLab
-
Albert, Thomas J. (author)
-
- Lundeberg, Joakim (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
-
- Sandberg, Rickard (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- 2015-08-03
- 2015
- English.
-
In: Genome Biology. - : Springer Science and Business Media LLC. - 1465-6906 .- 1474-760X. ; 16
- Related links:
-
https://genomebiolog...
-
show more...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Although the locations of promoters and enhancers have been identified in several cell types, we still have limited information on their connectivity. We developed HiCap, which combines a 4-cutter restriction enzyme Hi-C with sequence capture of promoter regions. Applying the method to mouse embryonic stem cells, we identified promoter-anchored interactions involving 15,905 promoters and 71,984 distal regions. The distal regions were enriched for enhancer marks and transcription, and had a mean fragment size of only 699 bp - close to single-enhancer resolution. High-resolution maps of promoter-anchored interactions with HiCap will be important for detailed characterizations of chromatin interaction landscapes.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database