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Non-contact protein...
Non-contact protein microarray fabrication using a procedure based on liquid bridge formation
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- Hartmann, Michael (författare)
- KTH,Analytisk kemi
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- Sjödahl, Johan (författare)
- KTH,Analytisk kemi
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- Stjernström, Mårten (författare)
- KTH,Analytisk kemi
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- Pettersson Redeby, Johan (författare)
- KTH,Analytisk kemi
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- Joos, Thomas (författare)
- NMI–Natural and Medical Sciences Institute at the University of Tübingen
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- Roeraade, Johan (författare)
- KTH,Analytisk kemi
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(creator_code:org_t)
- 2008-11-21
- 2009
- Engelska.
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Ingår i: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 393:2, s. 591-598
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Contemporary microarrayers of contact or noncontact format used in protein microarray fabrication still suffer from a number of problems, e. g. generation of satellite spots, inhomogeneous spots, misplaced or even absent spots, and sample carryover. In this paper, a new concept of non-contact sample deposition that reduces such problems is introduced. To show the potential and robustness of this pressure-assisted deposition technique, different sample solutions known to cause severe problems or to be even impossible to print with conventional microarrayers were accurately printed. The samples included 200 mg mL(-1) human serum albumin, highly concentrated sticky cell adhesion proteins, pure high-salt cell-lysis buffer, pure DMSO, and a suspension of 5-mu m polystyrene beads. Additionally, a water-immiscible liquid fluorocarbon, which was shown not to affect the functionality of the capture molecules, was employed as a lid to reduce evaporation during microarray printing. The fluorocarbon liquid lid was shown to circumvent hydrolysis of water-sensitive activated surfaces during long-term deposition procedures.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Analytical chemistry
- Analytisk kemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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