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Acoustic micro-vort...
Acoustic micro-vortexing of fluids, particles and cells in disposable microfluidic chips
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- Iranmanesh, Ida (författare)
- KTH,Tillämpad fysik
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- Ohlin, Mathias (författare)
- KTH,Tillämpad fysik
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- Ramachandraiah, Harisha (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Ye, Simon (författare)
- KTH,Tillämpad fysik
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- Russom, Aman (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Wiklund, Martin (författare)
- KTH,Biomedicinsk fysik och röntgenfysik
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(creator_code:org_t)
- 2016-07-21
- 2016
- Engelska.
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Ingår i: Biomedical microdevices (Print). - : Springer. - 1387-2176 .- 1572-8781. ; 18:4
- Relaterad länk:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We demonstrate an acoustic platform for microvortexing in disposable polymer microfluidic chips with small-volume (20 mu l) reaction chambers. The described method is demonstrated for a variety of standard vortexing functions, including mixing of fluids, re-suspension of a pellet of magnetic beads collected by a magnet placed on the chip, and lysis of cells for DNA extraction. The device is based on a modified Langevin-type ultrasonic transducer with an exponential horn for efficient coupling into the microfluidic chip, which is actuated by a low-cost fixed-frequency electronic driver board. The transducer is optimized by numerical modelling, and different demonstrated vortexing functions are realized by actuating the transducer for varying times; from fractions of a second for fluid mixing, to half a minute for cell lysis and DNA extraction. The platform can be operated during 1 min below physiological temperatures with the help of a PC fan, a Peltier element and an aluminum heat sink acting as the chip holder. As a proof of principle for sample preparation applications, we demonstrate on-chip cell lysis and DNA extraction within 25 s. The method is of interest for automating and chip-integrating sample preparation procedures in various biological assays.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
Nyckelord
- Acoustic streaming
- Lab on a chip
- Cell lysis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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