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Biodistribution of ...
Biodistribution of biodegradable polymeric nano-carriers loaded with busulphan and designed for multimodal imaging
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- Asem, Heba (författare)
- KTH,Funktionella material, FNM,Karolinska Institutet (KI), Sweden
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- Zhao, Ying (författare)
- Karolinska Institutet
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Ye, Fei (författare)
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Barrefelt, Asa (författare)
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- Abedi-Valugerdi, Manuchehr (författare)
- Karolinska Institutet
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El-Sayed, Ramy (författare)
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- El-Serafi, Ibrahim (författare)
- Karolinska Institutet
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Abu-Salah, Khalid M. (författare)
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Hamm, Jorg (författare)
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- Muhammed, Mamoun (författare)
- Karolinska Institutet,KTH,Funktionella material, FNM
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Hassan, Moustapha (författare)
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(creator_code:org_t)
- 2016-12-19
- 2016
- Engelska.
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Ingår i: Journal of Nanobiotechnology. - : BioMed Central (BMC). - 1477-3155. ; 14:1
- Relaterad länk:
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https://jnanobiotech...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
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- Background: Multifunctional nanocarriers for controlled drug delivery, imaging of disease development and follow-up of treatment efficacy are promising novel tools for disease diagnosis and treatment. In the current investigation, we present a multifunctional theranostic nanocarrier system for anticancer drug delivery and molecular imaging. Superparamagnetic iron oxide nanoparticles (SPIONs) as an MRI contrast agent and busulphan as a model for lipophilic antineoplastic drugs were encapsulated into poly (ethylene glycol)-co-poly (caprolactone) (PEG-PCL) micelles via the emulsion-evaporation method, and PEG-PCL was labelled with VivoTag 680XL fluorochrome for in vivo fluorescence imaging. Results: Busulphan entrapment efficiency was 83% while the drug release showed a sustained pattern over 10 h. SPION loaded-PEG-PCL micelles showed contrast enhancement in T-2*-weighted MRI with high r(2)* relaxivity. In vitro cellular uptake of PEG-PCL micelles labeled with fluorescein in J774A cells was found to be time-dependent. The maximum uptake was observed after 24 h of incubation. The biodistribution of PEG-PCL micelles functionalized with VivoTag 680XL was investigated in Balb/c mice over 48 h using in vivo fluorescence imaging. The results of real-time live imaging were then confirmed by ex vivo organ imaging and histological examination. Generally, PEG-PCL micelles were highly distributed into the lungs during the first 4 h post intravenous administration, then redistributed and accumulated in liver and spleen until 48 h post administration. No pathological impairment was found in the major organs studied. Conclusions: Thus, with loaded contrast agent and conjugated fluorochrome, PEG-PCL micelles as biodegradable and biocompatible nanocarriers are efficient multimodal imaging agents, offering high drug loading capacity, and sustained drug release. These might offer high treatment efficacy and real-time tracking of the drug delivery system in vivo, which is crucial for designing of an efficient drug delivery system.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Biodegradable polymer
- Drug delivery
- Magnetic resonance imaging
- In vivo fluorescence imaging
- Biodistribution
- Busulphan
- Cancer
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Asem, Heba
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Zhao, Ying
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Ye, Fei
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Barrefelt, Asa
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Abedi-Valugerdi, ...
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El-Sayed, Ramy
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visa fler...
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El-Serafi, Ibrah ...
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Abu-Salah, Khali ...
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Hamm, Jorg
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Muhammed, Mamoun
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Hassan, Moustaph ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Mikrobiologi
- Artiklar i publikationen
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Journal of Nanob ...
- Av lärosätet
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Kungliga Tekniska Högskolan
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Karolinska Institutet