Sökning: id:"swepub:oai:DiVA.org:kth-203763" >
The histone H2A iso...
The histone H2A isoform Hist2h2ac is a novel regulator of proliferation and epithelial-mesenchymal transition in mammary epithelial and in breast cancer cells.
-
Monteiro, Fátima Liliana (författare)
-
Vitorino, Rui (författare)
-
Wang, Jun (författare)
-
visa fler...
-
Cardoso, Hugo (författare)
-
Laranjeira, Hugo (författare)
-
Simões, Joana (författare)
-
Caldas, Margarida (författare)
-
Henrique, Rui (författare)
-
Amado, Francisco (författare)
-
- Williams, Cecilia, 1969- (författare)
- Karolinska Institutet,KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Cecilia Williams
-
Jerónimo, Carmen (författare)
-
Helguero, Luisa Ajav. (författare)
-
visa färre...
-
(creator_code:org_t)
- Elsevier, 2019
- 2019
- Engelska.
-
Ingår i: Cancer Letters. - : Elsevier. - 0304-3835 .- 1872-7980. ; 396, s. 42-52
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Proliferation and differentiation are controlled through chromatin remodelling. Therefore, there is an enormous biological significance and clinical value in understanding how specific signalling pathways are affected by histone replacement in the nucleosome. In this work, mass spectrometry was used to screen HC11 mammary epithelial cells for changes in histone levels throughout cell differentiation. The canonical histone isoform Histone H2A type 2-C (Hist2h2ac) was found only in undifferentiated/proliferating cells. Hist2h2ac mRNA was induced by EGF, specifically in the CD24+/CD29hi/DC44hi cell subpopulation. Hist2h2ac mRNA was increased by MEK(1/2) or PI3-K activation in HC11 and EpH4 mammary epithelial cells, and in MC4-L2 and T47-D breast cancer cells. Hist2h2ac silencing inhibited EGF-induced Zeb-1 expression and E-cadherin down-regulation, and this effect was reverted by Hist2h2ac re-expression. Notably, silencing of Hist2h2ac increased EGFR, ERBB2, and ERK(1/2) activation but did not allow EGF-induced proliferation. HIST2H2AC was expressed in all breast cancer molecular subtypes and found altered in 17% breast cancers, being 16.8% of the cases related to HIST2H2AC gene amplification and/or mRNA upregulation. In summary, this is the first study that identifies a canonical histone isoform -Hist2h2ac-downstream of the EGFR pathway, regulating oncogenic signalling and thereby contributing to deregulation of target genes.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Hist2h2ac
- Histone H2A variants
- breast cancer
- epidermal growth factor
- epithelial mesenchymal transition
- mammary epithelial cells
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Monteiro, Fátima ...
-
Vitorino, Rui
-
Wang, Jun
-
Cardoso, Hugo
-
Laranjeira, Hugo
-
Simões, Joana
-
visa fler...
-
Caldas, Margarid ...
-
Henrique, Rui
-
Amado, Francisco
-
Williams, Cecili ...
-
Jerónimo, Carmen
-
Helguero, Luisa ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
Cancer Letters
- Av lärosätet
-
Kungliga Tekniska Högskolan
-
Karolinska Institutet