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RNA activation-inde...
RNA activation-independent DNA targeting of the Type III CRISPR-Cas system by a Csm complex
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Park, Kwang-Hyun (author)
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An, Yan (author)
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- Jung, Taeyang (author)
- KTH,Strukturell bioteknik
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Baek, In-Young (author)
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Noh, Haemin (author)
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Ahn, Woo-Chan (author)
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- Hebert, Hans (author)
- Karolinska Institutet,KTH,Strukturell bioteknik
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Song, Ji-Joon (author)
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Kim, Jeong-Hoon (author)
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Oh, Byung-Ha (author)
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Woo, Eui-Jeon (author)
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(creator_code:org_t)
- 2017-03-31
- 2017
- English.
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In: EMBO Reports. - : Wiley-VCH Verlagsgesellschaft. - 1469-221X .- 1469-3178. ; 18:5, s. 826-840
- Related links:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The CRISPR-Cas system is an adaptive and heritable immune response that destroys invading foreign nucleic acids. The effector complex of the Type III CRISPR-Cas system targets RNA and DNA in a transcription-coupled manner, but the exact mechanism of DNA targeting by this complex remains elusive. In this study, an effector Csm holocomplex derived from Thermococcus onnurineus is reconstituted with a minimalistic combination of Csm1(1)2(1)3(3)4(1)5(1), and shows RNA targeting and RNA-activated single-stranded DNA (ssDNA) targeting activities. Unexpectedly, in the absence of an RNA transcript, it cleaves ssDNA containing a sequence complementary to the bound crRNA guide region in a manner dependent on the HD domain of the Csm1 subunit. This nuclease activity is blocked by a repeat tag found in the host CRISPR loci. The specific cleavage of ssDNA without a target RNA suggests a novel ssDNA targeting mechanism of the Type III system, which could facilitate the efficient and complete degradation of foreign nucleic acids.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- CRISPR
- Csm complex
- DNase
- RNase
- Thermococcus onnurineus
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Park, Kwang-Hyun
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An, Yan
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Jung, Taeyang
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Baek, In-Young
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Noh, Haemin
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Ahn, Woo-Chan
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show more...
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Hebert, Hans
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Song, Ji-Joon
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Kim, Jeong-Hoon
-
Oh, Byung-Ha
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Woo, Eui-Jeon
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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EMBO Reports
- By the university
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Royal Institute of Technology
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Karolinska Institutet