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The potential of au...
The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems
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Emmelkamp, J. (författare)
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Wolbers, F. (författare)
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- Andersson, Helene (författare)
- KTH,Bioteknologi
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DaCosta, R. S. (författare)
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Wilson, B. C. (författare)
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Vermes, I. (författare)
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van den Berg, A. (författare)
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(creator_code:org_t)
- Wiley, 2004
- 2004
- Engelska.
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Ingår i: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 25:21-22, s. 3740-3745
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A novel method for studying unlabeled living mammalian cells based on their autofluorescence (AF) signal in a prototype microfluidic device is presented. When combined, cellular AF detection and microfluidic devices have the potential to facilitate high-throughput analysis of different cell populations. To demonstrate this, unlabeled cultured cells in microfluidic devices were excited with a 488 nm excitation light and the AF emission (> 505 nm) was detected using a confocal fluorescence microscope (CFM). For example, a simple microfluidic three-port glass microstructure was used together with conventional electroosmotic flow (EOF) to switch the direction of the fluid flow. As a means to test the potential of AF-based cell sorting in this microfluidic device, granulocytes were successfully differentiated from human red blood cells (RBCs) based on differences in AF This study demonstrated the use of a simple microfabricated device to perform high-throughput live cell detection and differentiation without the need for cell-specific fluorescent labeling dyes and thereby reducing the sample preparation time. Hence, the combined use of microfluidic devices and cell AF may have many applications in single-cell analysis.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- autofluorescence
- microfluidic
- miniaturization
- red blood cell
- single-cell analysis
- spectroscopy
- transport
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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