Dissecting the spatiotemporal subcellular distribution of the human proteome

• Compartmentalization of biological reactions is an important mechanism to allow multiple cellular reactions to occur in parallel. Resolving the spatial distribution of the human proteome at a subcellular level increases our understanding of human biology and disease. We have generated a high-resolution map of the subcellular distribution of the human proteome as part of the open access Human Protein Atlas database. We have shown that as much as half of all proteins localize to multiple compartments. Such proteins may have context specific functions and ‘moonlight’ in different parts of the cell, thus increasing the functionality of the proteome and the complexity of the cell from a systems perspective. I will present how this spatial data can complement quantitative omics data for improved functional read-out. Furthermore, I will present unpublished data on the extent of single cell variations of the human proteome, in correlation to cell cycle progression and other deterministic factors, as well as the overlap with observed variations at the RNA level. In summary, I will demonstrate the importance of spatial proteomics data for improved single cell biology.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

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