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Substrate Elasticit...
Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture
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Gilbert, P. M. (author)
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Havenstrite, K. L. (author)
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- Magnusson, Klas E. G. (author)
- KTH,Signalbehandling
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Sacco, A. (author)
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Leonardi, N. A. (author)
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Kraft, P. (author)
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Nguyen, N. K. (author)
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Thrun, S. (author)
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Lutolf, M. P. (author)
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Blau, H. M. (author)
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(creator_code:org_t)
- American Association for the Advancement of Science (AAAS), 2010
- 2010
- English.
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In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 329:5995, s. 1078-1081
- Related links:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Stem cells that naturally reside in adult tissues, such as muscle stem cells (MuSCs), exhibit robust regenerative capacity in vivo that is rapidly lost in culture. Using a bioengineered substrate to recapitulate key biophysical and biochemical niche features in conjunction with a highly automated single-cell tracking algorithm, we show that substrate elasticity is a potent regulator of MuSC fate in culture. Unlike MuSCs on rigid plastic dishes (similar to 10(6) kilopascals), MuSCs cultured on soft hydrogel substrates that mimic the elasticity of muscle (12 kilopascals) self-renew in vitro and contribute extensively to muscle regeneration when subsequently transplanted into mice and assayed histologically and quantitatively by noninvasive bioluminescence imaging. Our studies provide novel evidence that by recapitulating physiological tissue rigidity, propagation of adult muscle stem cells is possible, enabling future cell-based therapies for muscle-wasting diseases.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- Cell biology
- Cellbiologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Gilbert, P. M.
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Havenstrite, K. ...
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Magnusson, Klas ...
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Sacco, A.
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Leonardi, N. A.
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Kraft, P.
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show more...
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Nguyen, N. K.
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Thrun, S.
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Lutolf, M. P.
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Blau, H. M.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
- Articles in the publication
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Science
- By the university
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Royal Institute of Technology