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Cell Cycle Profilin...
Cell Cycle Profiling Reveals Protein Oscillation, Phosphorylation, and Localization Dynamics
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- Herr, Patrick (författare)
- Karolinska Inst, Dept Oncol Pathol, Sci Life Lab, S-17176 Stockholm, Sweden.;Univ Sheffield, Dept Oncol & Metab, Weston Pk Canc Ctr, Sheffield S10 2RX, S Yorkshire, England.
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- Boström, Johan (författare)
- Karolinska Institutet
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- Rullman, Eric (författare)
- Karolinska Institutet
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- Rudd, Sean G. (författare)
- Karolinska Institutet
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- Vesterlund, Mattias (författare)
- Karolinska Institutet
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- Lehtio, Janne (författare)
- Karolinska Institutet
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- Helleday, Thomas (författare)
- Karolinska Institutet
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- Maddalo, Gianluca (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Altun, Mikael (författare)
- Karolinska Institutet
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Karolinska Institutet Karolinska Inst, Dept Oncol Pathol, Sci Life Lab, S-17176 Stockholm, Sweden;Univ Sheffield, Dept Oncol & Metab, Weston Pk Canc Ctr, Sheffield S10 2RX, S Yorkshire, England. (creator_code:org_t)
- Elsevier BV, 2020
- 2020
- Engelska.
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Ingår i: Molecular & Cellular Proteomics. - : Elsevier BV. - 1535-9476 .- 1535-9484. ; 19:4, s. 608-623
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- The cell cycle is a highly conserved process involving the coordinated separation of a single cell into two daughter cells. To relate transcriptional regulation across the cell cycle with oscillatory changes in protein abundance and activity, we carried out a proteome- and phospho-proteome-wide mass spectrometry profiling. We compared protein dynamics with gene transcription, revealing many transcriptionally regulated G2 mRNAs that only produce a protein shift after mitosis. Integration of CRISPR/Cas9 survivability studies further highlighted proteins essential for cell viability. Analyzing the dynamics of phosphorylation events and protein solubility dynamics over the cell cycle, we characterize predicted phospho-peptide motif distributions and predict cell cycle-dependent translocating proteins, as exemplified by the S-adenosylmethionine synthase MAT2A. Our study implicates this enzyme in translocating to the nucleus after the G1/S-checkpoint, which enables epigenetic histone methylation maintenance during DNA replication. Taken together, this data set provides a unique integrated resource with novel insights on cell cycle dynamics.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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