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A Coculture Based T...
A Coculture Based Tyrosine-Tyrosinase Electrochemical Gene Circuit for Connecting Cellular Communication with Electronic Networks
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- VanArsdale, Eric (författare)
- Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA.
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- Hörnström, David (författare)
- KTH,Industriell bioteknologi
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- Sjöberg, Gustav (författare)
- KTH,Industriell bioteknologi
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- Järbur, Ida (författare)
- KTH,Industriell bioteknologi
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- Pitzer, Juliana (författare)
- Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA.
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- Payne, Gregory F. (författare)
- Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA.
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- van Maris, Antonius J. A., Professor, 1976- (författare)
- KTH,Industriell bioteknologi
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- Bentley, William E. (författare)
- Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA.
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Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA Industriell bioteknologi (creator_code:org_t)
- 2020-03-25
- 2020
- Engelska.
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Ingår i: ACS Synthetic Biology. - : American Chemical Society (ACS). - 2161-5063. ; 9:5, s. 1117-1128
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- There is a growing interest in mediating information transfer between biology and electronics. By the addition of redox mediators to various samples and cells, one can both electronically obtain a redox "portrait" of a biological system and, conversely, program gene expression. Here, we have created a cell-based synthetic biology-electrochemical axis in which engineered cells process molecular cues, producing an output that can be directly recorded via electronics-but without the need for added redox mediators. The process is robust; two key components must act together to provide a valid signal. The system builds on the tyrosinase-mediated conversion of tyrosine to L-DOPA and L-DOPAquinone, which are both redox active. "Catalytic" transducer cells provide for signal-mediated surface expression of tyrosinase. Additionally, "reagent" transducer cells synthesize and export tyrosine, a substrate for tyrosinase. In cocultures, this system enables real-time electrochemical transduction of cell activating molecular cues. To demonstrate, we eavesdrop on quorum sensing signaling molecules that are secreted by Pseudomonas aeruginosa, N-(3-oxododecanoyl)-l-homoserine lactone and pyocyanin.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
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