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Live Cell FRET Imag...
Live Cell FRET Imaging Reveals Amyloid beta-Peptide Oligomerization in Hippocampal Neurons
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- Gao, Yang (författare)
- Karolinska Inst, Dept Neurobiol Care Sci & Soc, Div Neurogeriatr, S-17164 Solna, Sweden.
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- Wennmalm, Stefan, 1970- (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Biofysik
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- Winblad, Bengt (författare)
- Karolinska Inst, Dept Neurobiol Care Sci & Soc, Div Neurogeriatr, S-17164 Solna, Sweden.;Karolinska Univ Hosp, Theme Inflammat & Aging, S-14186 Huddinge, Sweden.
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- Schedin-Weiss, Sophia (författare)
- Karolinska Inst, Dept Neurobiol Care Sci & Soc, Div Neurogeriatr, S-17164 Solna, Sweden.
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- Tjernberg, Lars O. (författare)
- Karolinska Inst, Dept Neurobiol Care Sci & Soc, Div Neurogeriatr, S-17164 Solna, Sweden.
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Karolinska Inst, Dept Neurobiol Care Sci & Soc, Div Neurogeriatr, S-17164 Solna, Sweden Science for Life Laboratory, SciLifeLab (creator_code:org_t)
- 2021-04-26
- 2021
- Engelska.
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Ingår i: International Journal of Molecular Sciences. - : MDPI AG. - 1661-6596 .- 1422-0067. ; 22:9
- Relaterad länk:
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https://doi.org/10.3...
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Amyloid beta-peptide (A beta) oligomerization is believed to contribute to the neuronal dysfunction in Alzheimer disease (AD). Despite decades of research, many details of A beta oligomerization in neurons still need to be revealed. Forster resonance energy transfer (FRET) is a simple but effective way to study molecular interactions. Here, we used a confocal microscope with a sensitive Airyscan detector for FRET detection. By live cell FRET imaging, we detected A beta 42 oligomerization in primary neurons. The neurons were incubated with fluorescently labeled A beta 42 in the cell culture medium for 24 h. A beta 42 were internalized and oligomerized in the lysosomes/late endosomes in a concentration-dependent manner. Both the cellular uptake and intracellular oligomerization of A beta 42 were significantly higher than for A beta 40. These findings provide a better understanding of A beta 42 oligomerization in neurons.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- amyloid β
- -peptide
- oligomerization
- aggregation
- FRET
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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