High-content nucleus based 3D image cytometery of whole multicellular tumour spheroids

• We have previously presented an ultrasonic standing wave (USW) based multicellular tumor spheroid (MCTS) culture platform where 100 MCST can be formed, processed and imaged while being retained in micro-wells [1]. This platform was used to show MCTS characterization based on on-chip microscopy and off-chip flow cytometry [2]. Here, we present a high-content 3D image cytometry method of whole MCTS based on DNA staining where spatial and functional information can be assessed.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Nyckelord

Confocal imaging

High-content cytometry

Image analysis

Multicellular tumor spheroids

3-D image

Cytometry

Functional information

High-content

Micro wells

Ultrasonic standing waves

Tumors

Publikations- och innehållstyp

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