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Exploiting Electros...
Exploiting Electrostatic Interaction for Highly Sensitive Detection of Tumor-Derived Extracellular Vesicles by an Electrokinetic Sensor
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- Sahu, Siddharth S. (författare)
- Uppsala universitet,Fasta tillståndets elektronik
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- Cavallaro, Sara (författare)
- KTH,Fotonik,KTH Royal Inst Technol, Sch Engn Sci, Dept Appl Phys, S-10691 Stockholm, Sweden.
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- Haag, Petra (författare)
- Karolinska Institutet
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- Nagy, Abel (författare)
- KTH,Proteinvetenskap,KTH Royal Inst Technol, Sch Chem Biotechnol & Hlth CBH, Dept Prot Sci, S-10691 Stockholm, Sweden.
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- Eriksson Karlström, Amelie (författare)
- KTH,Proteinvetenskap,KTH Royal Inst Technol, Sch Chem Biotechnol & Hlth CBH, Dept Prot Sci, S-10691 Stockholm, Sweden.
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- Lewensohn, Rolf (författare)
- Karolinska Institutet
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- Viktorsson, Kristina (författare)
- Karolinska Institutet
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- Linnros, Jan, 1953- (författare)
- KTH,Fotonik,KTH Royal Inst Technol, Sch Engn Sci, Dept Appl Phys, S-10691 Stockholm, Sweden.
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- Dev, Apurba (författare)
- Uppsala universitet,KTH,Fotonik,Uppsala Univ, Dept Elect Engn, Angstrom Lab, S-75121 Uppsala, Sweden.,Fasta tillståndets elektronik,KTH Royal Inst Technol, Sch Engn Sci, Dept Appl Phys, S-10691 Stockholm, Sweden.
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(creator_code:org_t)
- 2021-09-02
- 2021
- Engelska.
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Ingår i: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 13:36, s. 42513-42521
- Relaterad länk:
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https://doi.org/10.1...
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https://pubs.acs.org...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- We present an approach to improve the detection sensitivity of a streaming current-based biosensor for membrane protein profiling of small extracellular vesicles (sEVs). The experimental approach, supported by theoretical investigation, exploits electrostatic charge contrast between the sensor surface and target analytes to enhance the detection sensitivity. We first demonstrate the feasibility of the approach using different chemical functionalization schemes to modulate the zeta potential of the sensor surface in a range -16.0 to -32.8 mV. Thereafter, we examine the sensitivity of the sensor surface across this range of zeta potential to determine the optimal functionalization scheme. The limit of detection (LOD) varied by 2 orders of magnitude across this range, reaching a value of 4.9 x 10(6) particles/mL for the best performing surface for CD9. We then used the optimized surface to profile CD9, EGFR, and PD-L1 surface proteins of sEVs derived from non-small cell lung cancer (NSCLC) cell-line H1975, before and after treatment with EGFR tyrosine kinase inhibitors, as well as sEVs derived from pleural effusion fluid of NSCLC adenocarcinoma patients. Our results show the feasibility to monitor CD9, EGFR, and PD-L1 expression on the sEV surface, illustrating a good prospect of the method for clinical application.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- streaming current
- electrokinetic method
- charge modulation
- enhanced sensitivity
- extracellular vesicles
- surface proteins
- lung cancer
- treatment monitoring
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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