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Event-triggered STE...
Event-triggered STED imaging
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- Alvelid, Jonatan (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab,Ilaria Testa
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- Damenti, Martina (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab,Ilaria Testa
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- Testa, Ilaria (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- Cold Spring Harbor Laboratory, 2024
- Engelska.
- Relaterad länk:
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https://www.biorxiv....
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visa fler...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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visa färre...
Abstract
Ämnesord
Stäng
- The observation of protein organization during cellular signalling calls for imaging methods with increased spatial and temporal resolution. STED nanoscopy can access dynamics of nanoscale structures in living cells. However, the available number of recordable frames is often limited by photo-bleaching. Here, we present an automated method, event-triggered STED, which instantly (< 40 ms) images synaptic proteins with high spatial and temporal resolution (~30 nm, 2.5 Hz) in small regions upon and at the site of local calcium sensing.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- STED
- microscopy
- nanoscopy
- automation
- Biological Physics
- Biologisk fysik
- Optik och fotonik
- Optics and Photonics
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)