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Rapid and sensitive...
Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis
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- Hober, Andreas (författare)
- KTH,Science for Life Laboratory, SciLifeLab
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- Hua, Tranh-Min Khue (författare)
- KTH,Systembiologi
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- Foley, Dominic (författare)
- Waters Corp, Milford, England.
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- McDonald, Thomas (författare)
- Waters Corp, Milford, England.
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- Vissers, Johannes Pc (författare)
- Waters Corp, Milford, England.
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- Pattison, Rebecca (författare)
- Waters Corp, Milford, England.
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- Ferries, Samantha (författare)
- Waters Corp, Milford, England.
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- Hermansson, Sigurd (författare)
- Waters Corp, Milford, England.
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- Betner, Ingvar (författare)
- Waters Corp, Milford, England.
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- Uhlén, Mathias (författare)
- KTH,Systembiologi
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- Razavi, Morteza (författare)
- SISCAPA Assay Technol Inc, Victoria, BC, Canada.
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- Yip, Richard (författare)
- SISCAPA Assay Technol Inc, Victoria, BC, Canada.
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- Pope, Matthew E. (författare)
- SISCAPA Assay Technol Inc, Victoria, BC, Canada.
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- Pearson, Terry W. (författare)
- SISCAPA Assay Technol Inc, Victoria, BC, Canada.
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- Andersson, Leigh N. (författare)
- SISCAPA Assay Technol Inc, Victoria, BC, Canada.
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- Bartlett, Amy (författare)
- Waters Corp, Milford, England.
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- Calton, Lisa (författare)
- Waters Corp, Milford, England.
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- Alm, Jessica J. (författare)
- Karolinska Institutet
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- Engstrand, Lars (författare)
- Karolinska Institutet
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- Edfors, Fredrik (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Systembiologi
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(creator_code:org_t)
- eLIFE SCIENCES PUBL LTD, 2021
- 2021
- Engelska.
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Ingår i: eLIFE. - : eLIFE SCIENCES PUBL LTD. - 2050-084X. ; 10
- Relaterad länk:
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https://doi.org/10.7...
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https://urn.kb.se/re...
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https://doi.org/10.7...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Reliable, robust, large-scale molecular testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for monitoring the ongoing coronavirus disease 2019 (COVID-19) pandemic. We have developed a scalable analytical approach to detect viral proteins based on peptide immuno-affinity enrichment combined with liquid chromatography-mass spectrometry (LC-MS). This is a multiplexed strategy, based on targeted proteomics analysis and read-out by LC-MS, capable of precisely quantifying and confirming the presence of SARS-CoV-2 in phosphate-buffered saline (PBS) swab media from combined throat/nasopharynx/saliva samples. The results reveal that the levels of SARS-CoV-2 measured by LC-MS correlate well with their correspondingreal-time polymerase chain reaction (RT-PCR) read-out (r = 0.79). The analytical workflow shows similar turnaround times as regular RT-PCR instrumentation with a quantitative read-out of viral proteins corresponding to cycle thresholds (Ct) equivalents ranging from 21 to 34. Using RT-PCR as a reference, we demonstrate that the LC-MS-based method has 100% negative percent agreement (estimated specificity) and 95% positive percent agreement (estimated sensitivity) when analyzing clinical samples collected from asymptomatic individuals with a Ct within the limit of detection of the mass spectrometer (Ct <= 30). These results suggest that a scalable analytical method based on LC-MS has a place in future pandemic preparedness centers to complement current virus detection technologies.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Nyckelord
- SARS CoV-2
- COVID-19
- SISCAPA
- proteomics
- diagnostics
- mass spectrometry
- Human
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
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eLIFE
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Till lärosätets databas
- Av författaren/redakt...
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Hober, Andreas
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Hua, Tranh-Min K ...
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Foley, Dominic
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McDonald, Thomas
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Vissers, Johanne ...
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Pattison, Rebecc ...
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visa fler...
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Ferries, Samanth ...
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Hermansson, Sigu ...
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Betner, Ingvar
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Uhlén, Mathias
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Razavi, Morteza
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Yip, Richard
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Pope, Matthew E.
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Pearson, Terry W ...
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Andersson, Leigh ...
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Bartlett, Amy
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Calton, Lisa
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Alm, Jessica J.
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Engstrand, Lars
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Edfors, Fredrik
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Infektionsmedici ...
- Artiklar i publikationen
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eLIFE
- Av lärosätet
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Kungliga Tekniska Högskolan
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Karolinska Institutet