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Proof-of-Concept of...
Proof-of-Concept of a Novel Cell Separation Technology Using Magnetic Agarose-Based Beads
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- Brechmann, Nils A. (författare)
- KTH,Industriell bioteknologi,VINNOVA Competence Ctr Adv Bioprod Continuous Pro, AdBIOPRO, SE-11421 Stockholm, Sweden.,Grp CETEG,KTH Royal Inst Technol, Sweden; KTH Royal Inst Technol, Sweden
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- Jansson, Marta (författare)
- AstraZeneca, BioPharmaceut R&D Cell Therapy Dept, Res & Early Dev, Cardiovasc Renal & Matabolism CVRM,BioPharmaceut, SE-43183 Gothenburg, Sweden.
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- Hagg, Alice (författare)
- AstraZeneca, BioPharmaceut R&D Cell Therapy Dept, Res & Early Dev, Cardiovasc Renal & Matabolism CVRM,BioPharmaceut, SE-43183 Gothenburg, Sweden.
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- Hicks, Ryan (författare)
- AstraZeneca, BioPharmaceut R&D Cell Therapy Dept, Res & Early Dev, Cardiovasc Renal & Matabolism CVRM,BioPharmaceut, SE-43183 Gothenburg, Sweden.
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- Hyllner, Johan (författare)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten,AstraZeneca, Sweden
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- Eriksson, Kristofer (författare)
- MAG Bioproc, SE-75450 Uppsala, Sweden.
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- Chotteau, Véronique, Docent, 1963- (författare)
- KTH,Industriell bioteknologi,AdBIOPRO, SE-11421 Stockholm, Sweden.,KTH Royal Inst Technol, Sweden; KTH Royal Inst Technol, Sweden
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(creator_code:org_t)
- 2022-03-10
- 2022
- Engelska.
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Ingår i: MAGNETOCHEMISTRY. - : MDPI AG. - 2312-7481. ; 8:3, s. 34-
- Relaterad länk:
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https://doi.org/10.3...
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https://kth.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The safety of the cells used for Advanced Therapy Medicinal Products is crucial for patients. Reliable methods for the cell purification are very important for the commercialization of those new therapies. With the large production scale envisioned for commercialization, the cell isolation methods need to be efficient, robust, operationally simple and generic while ensuring cell biological functionality and safety. In this study, we used high magnetized magnetic agarose-based beads conjugated with protein A to develop a new method for cell separation. A high separation efficiency of 91% yield and consistent isolation performances were demonstrated using population mixtures of human mesenchymal stem cells and HER2(+) SKBR3 cells (80:20, 70:30 and 30:70). Additionally, high robustness against mechanical stress and minimal unspecific binding obtained with the protein A base conjugated magnetic beads were significant advantages in comparison with the same magnetic microparticles where the antibodies were covalently conjugated. This study provided insights on features of large high magnetized microparticles, which is promising for the large-scale application of cell purification.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- cell separation
- magnetic bead separation
- cell sorting
- HER2(+)
- SKBR3 cells
- hMSCs
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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