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Capillary and micro...
Capillary and microchip electrophoresis method development for amino acid monitoring during biopharmaceutical cultivation
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- Mikkonen, Saara (författare)
- KTH,Tillämpad fysikalisk kemi,Xbrane Biopharma, Stockholm, Sweden
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- Josefsson, Leila (författare)
- KTH,Tillämpad fysikalisk kemi,Kantisto BV, Baarn, Netherlands
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- Mäkinen, Meeri E.-L. (författare)
- KTH,Industriell bioteknologi
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- Chotteau, Veronique, Docent, 1963- (författare)
- KTH,Industriell bioteknologi
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- Emmer, Åsa (författare)
- KTH,Tillämpad fysikalisk kemi
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(creator_code:org_t)
- 2022-05-13
- 2022
- Engelska.
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Ingår i: Biotechnology Journal. - : Wiley. - 1860-6768 .- 1860-7314.
- Relaterad länk:
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https://doi.org/10.1...
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https://kth.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The increased use of biopharmaceuticals calls for improved means of bioprocess monitoring. In this work, capillary electrophoresis (CE) and microchip electrophoresis (MCE) methods were developed and applied for the analysis of amino acids (AAs) in cell culture supernatant. In samples from different days of a Chinese hamster ovary cell cultivation process, all 19 proteinogenic AAs containing primary amine groups could be detected using CE, and 17 out of 19 AAs using MCE. The relative concentration changes in different samples agreed well with those measured by high-performance liquid chromatography (HPLC). Compared to the more commonly employed HPLC analysis, the CE and MCE methods resulted in faster analysis, while significantly lowering both the sample and reagent consumption, and the cost per analysis.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- amino acid
- bioprocess monitoring
- capillary electrophoresis
- cell culture
- microchip electrophoresis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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