Multiomics Analysis Reveals the Impact of Microbiota on Host Metabolism in Hepatic Steatosis

• Metabolic dysfunction-associated fatty liver disease (MAFLD) is a complex disease involving alterations in multiple biological processes regulated by the interactions between obesity, genetic background, and environmental factors including the microbiome. To decipher hepatic steatosis (HS) pathogenesis by excluding critical confounding factors including genetic variants and diabetes, 56 heterogenous MAFLD patients are characterized by generating multiomics data including oral and gut metagenomics as well as plasma metabolomics and inflammatory proteomics data. The dysbiosis in the oral and gut microbiome is explored and the host–microbiome interactions based on global metabolic and inflammatory processes are revealed. These multiomics data are integrated using the biological network and HS's key features are identified using multiomics data. HS is finally predicted using these key features and findings are validated in a follow-up cohort, where 22 subjects with varying degree of HS are characterized.

Ämnesord

NATURVETENSKAP  -- Biologi -- Bioinformatik och systembiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Medical Genetics (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

Nyckelord

gut and oral metagenomics

metabolic dysfunction-associated fatty liver disease

metabolomics

multiomics analysis

proteomics

systems biology

systems medicine

Diseases

Metabolism

Fatty liver disease

Gut and oral metagenomic

Hepatic steatosis

Metagenomics

Microbiome

Multiomic analyse

System medicine

Molecular biology

dysbiosis

fatty liver

genetics

human

intestine flora

microflora

Gastrointestinal Microbiome

Humans

Microbiota

gut and oral metagenomics

metabolic dysfunction-associated fatty liver disease

metabolomics

multiomics analysis

proteomics

systems biology

systems medicine

Diseases

Metabolism

Fatty liver disease

Gut and oral metagenomic

Hepatic steatosis

Metagenomics

Microbiome

Multiomic analyse

System medicine

Molecular biology

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