Sökning: id:"swepub:oai:DiVA.org:kth-335453" >
Cloning, characteri...
Cloning, characterization and impact of up- and down-regulating subabul cinnamyl alcohol dehydrogenase (CAD) gene on plant growth and lignin profiles in transgenic tobacco
-
Sirisha, V. L. (författare)
-
Prashant, S (författare)
-
Ranadheer Kumar, D. (författare)
-
visa fler...
-
Sivan, Pramod, 1984- (författare)
-
Jalaja, N. (författare)
-
Hima Kumari, P. (författare)
-
Maheshwari Rao, P. (författare)
-
Nageswara Rao, S. (författare)
-
Mishra, Preeti (författare)
-
Rao Karumanchi, S. (författare)
-
Khan, B. M. (författare)
-
Kavi Kishor, P. B. (författare)
-
visa färre...
- 2011-12-29
- 2011
- Engelska.
-
Ingår i: Plant growth regulation (Print). - : Springer Nature. - 0167-6903 .- 1573-5087. ; 66:3, s. 239-253
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Both cDNA including 5′UTR and 3′UTR and genomic clones of cinnamyl alcohol dehydrogenase (CAD) were isolated and characterized from a pulp-yielding leguminous tree Leucaena leucocephala (LlCAD1). The deduced amino acid sequence shared high identity with orthologous sequences of Acacia mangium × Acacia auriculiformis (83%), Medicago sativa (83%), Nicotiana tabaccum (83%) and Aralia cordata (81%). Full length cDNA contained 78 bases of 5′UTR and 283 bases of 3′UTR, while the genomic clone contained 5 exons and 4 introns. Western blot analysis revealed elevated expression of LlCAD1 in seedling roots and shoots compared to leaves. Sense and antisense CAD tobacco transgenics showed increased and reduced CAD activity accompanied by a change in monomeric lignin composition. Histochemical staining of lignin in down-regulated plants suggested an increase in aldehyde units and a decrease in S/G ratio. Down-regulation of CAD resulted in accumulation of syringic, ferulic, p-coumaric and sinapic acids compared to untransformed controls. These observations were validated by anatomical studies of down-regulated transgenic stems which showed thin walled, elongated phloem and xylem fibres, accompanied by a reduction in the density of vessel elements and amount of secondary xylem when compared to untransformed plants. Furthermore, Klason lignin analysis of CAD antisense transgenics showed 7–32% reduced lignin and normal phenotype as compared to untransformed plants. Such a reduction was not noticed in up-regulated transgenics. These results demonstrate a unique opportunity to explore the significant role that down-regulation of CAD gene plays in reducing lignin content thereby offering potential benefits to the pulp and paper industry.
Nyckelord
- Biotechnology
- Bioteknologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Sirisha, V. L.
-
Prashant, S
-
Ranadheer Kumar, ...
-
Sivan, Pramod, 1 ...
-
Jalaja, N.
-
Hima Kumari, P.
-
visa fler...
-
Maheshwari Rao, ...
-
Nageswara Rao, S ...
-
Mishra, Preeti
-
Rao Karumanchi, ...
-
Khan, B. M.
-
Kavi Kishor, P. ...
-
visa färre...
- Artiklar i publikationen
-
Plant growth reg ...
- Av lärosätet
-
Kungliga Tekniska Högskolan