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Fast Depolymerizati...
Fast Depolymerization of PET Bottle Mediated by Microwave Pre-Treatment and An Engineered PETase
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- Guo, Boyang (författare)
- KTH,Ytbehandlingsteknik,Science for Life Laboratory, SciLifeLab
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- Lopez-Lorenzo, Ximena (författare)
- KTH,Ytbehandlingsteknik,Science for Life Laboratory, SciLifeLab
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- Fang, Yuan (författare)
- KTH,Tillämpad fysikalisk kemi
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- Bäckström, Eva (författare)
- KTH,Polymerteknologi
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- Capezza, Antonio Jose (författare)
- KTH,Polymera material
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- Vanga, Sudarsana Reddy (författare)
- KTH,Ytbehandlingsteknik,Science for Life Laboratory, SciLifeLab
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- Furo, Istvan (författare)
- KTH,Tillämpad fysikalisk kemi
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- Hakkarainen, Minna (författare)
- KTH,Polymerteknologi
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- Syrén, Per-Olof (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Ytbehandlingsteknik
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(creator_code:org_t)
- Wiley, 2023
- 2023
- Engelska.
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Ingår i: ChemSusChem. - : Wiley. - 1864-5631 .- 1864-564X. ; 16:18
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Recycling plastics is the key to reaching a sustainable materials economy. Biocatalytic degradation of plastics shows great promise by allowing selective depolymerization of man-made materials into constituent building blocks under mild aqueous conditions. However, insoluble plastics have polymer chains that can reside in different conformations and show compact secondary structures that offer low accessibility for initiating the depolymerization reaction by enzymes. In this work, we overcome these shortcomings by microwave irradiation as a pre-treatment process to deliver powders of polyethylene terephthalate (PET) particles suitable for subsequent biotechnology-assisted plastic degradation by previously generated engineered enzymes. An optimized microwave step resulted in 1400 times higher integral of released terephthalic acid (TPA) from high-performance liquid chromatography (HPLC), compared to original untreated PET bottle. Biocatalytic plastic hydrolysis of substrates originating from PET bottles responded to 78 % yield conversion from 2 h microwave pretreatment and 1 h enzymatic reaction at 30 °C. The increase in activity stems from enhanced substrate accessibility from the microwave step, followed by the administration of designer enzymes capable of accommodating oligomers and shorter chains released in a productive conformation.
Ämnesord
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Livsmedelsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Food Science (hsv//eng)
Nyckelord
- enzyme engineering
- microwave pre-treatment
- plastic biodegradation
- polyesterase
- polyethylene terephthalate recycling
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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