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Quantitative and fu...
Quantitative and functional assessment of Arc n-meric states in membrane interaction and AMPA receptor endocytosis
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- Damenti, Martina (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab,Ilaria Testa
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- Coceano, Giovanna (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab,Ilaria Testa
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- Mendes Silva, Mariline (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Biofysik,Ilaria Testa
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visa fler...
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- Alvelid, Jonatan (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab,Ilaria Testa
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- Sgattoni, Chiara (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab,Ilaria Testa
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- Rems, Lea (författare)
- SciLifeLab, Stockholm University, Stockholm, Sweden,Lucie Delemotte
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- Sezgin, Erdinc (författare)
- Department of Women's and Children's Health, Karolinska Institutet, 171 77, Stockholm, Sweden,Erdinc Sezgin
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- Unterauer, Eduard (författare)
- Max Plank Institute of Biochemistry, 82152 Planegg, Germany,Ralf Jungmann
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- Kowalewski, Rafal (författare)
- Max Plank Institute of Biochemistry, 82152 Planegg, Germany,Ralf Jungmann
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- Volpato, Andrea (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab,Ilaria Testa
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- Delemotte, Lucie (författare)
- SciLifeLab, Stockholm University, Stockholm, Sweden,Lucie Delemotte
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- Masullo, Luciano (författare)
- Max Plank Institute of Biochemistry, 82152 Planegg, Germany,Ralf Jungmann
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- Jungmann, Ralf (författare)
- Max Plank Institute of Biochemistry, 82152 Planegg, Germany,Ralf Jungmann
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- Testa, Ilaria (författare)
- KTH,Biofysik,Science for Life Laboratory, SciLifeLab,Ilaria Testa
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Arc (or Arg3.1), Activity Regulated-Cytoskeleton associated-protein is pivotal to mediate plastic responses in neuronal cells. In vitro and in vivo studies suggest its ability to form high- and low-order oligomers which are potentially involved in neuronal trafficking. Despite its important function, no direct observation of Arc oligomers in cells has been presented due to its highly regulated spatiotemporal expression, the small size of the structures, the lack of appropriate labelling strategies and the background associated to free diffusing cytosolic proteins. Here, we take advantage of several complementary advanced fluorescence microscopy and spectroscopy techniques to observe and quantify Arc oligomeric states in cellular environment especially in the synapses. In cells, we uncovered Arc-Arc intermolecular interactions, Arc tendency to form liquid condensates and to interact with lipid bilayers. High-order oligomers are found to localize at the excitatory synaptic compartment and to directly affects AMPA receptor surface levels. Together, our observations support the model by which Arc oligomerization mediates plasma- membrane negative inward curvature favoring AMPA receptors endocytosis.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Arc (Activity-regulated cytoskeleton-associated protein)
- AMPA receptors
- oligomerization
- STED
- FCS
- DNA-PAINT
- STARSS
- molecular dynamics simulations.
- Biological and Biomedical Physics
- Biologisk och biomedicinsk fysik
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)
- Av författaren/redakt...
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Damenti, Martina
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Coceano, Giovann ...
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Mendes Silva, Ma ...
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Alvelid, Jonatan
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Sgattoni, Chiara
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Rems, Lea
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visa fler...
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Sezgin, Erdinc
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Unterauer, Eduar ...
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Kowalewski, Rafa ...
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Volpato, Andrea
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Delemotte, Lucie
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Masullo, Luciano
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Jungmann, Ralf
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Testa, Ilaria
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
- Av lärosätet
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Kungliga Tekniska Högskolan