Molecular basis of perhydrolase activity in serine hydrolases

• (Chemical Equation Presented) Changing substrates: A mutation that forms a cis-proline-peptide bond in a loop structure close to the active site of an aryl esterase from Pseudomonas fluorescens converts the enzyme into a perhydrolase (see picture). The switch in activity is explained by a new hydrogen bond formed between a backbone carbonyl oxygen atom and the peroxy deacylation intermediate.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

enzyme catalysis

hydrolases

molecular modeling

mutagenesis

peroxides

Biochemistry

Biokemi

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)