Ligand-receptor interactions and membrane structure investigated by AFM and time-resolved fluorescence microscopy

• The atomic force microscope (AFM) and the associated dynamic force spectroscopy technique have been exploited to quantitatively assess the interaction between proteins and their binding to specific ligands and membrane surfaces. In particular, we have studied the specific interaction between lung surfactant protein D and various carbohydrates. In addition, we have used scanning AFM and time-resolved fluorescence microscopy to image the lateral structure of different lipid bilayers and their morphological changes as a function of time. The various systems studied illustrate the potential of modern AFM techniques for application to biomedical research, specifically within immunology and liposome-based drug delivery.

Nyckelord

Atomic force microscopy

Force spectroscopy

Lateral membrane structure

Ligand-receptor interaction

Phospholipase A2

Single molecules

Surfactant protein D

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