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Connector Inversion...
Connector Inversion Probe Technology : A Powerful One- Primer Multiplex DNA Amplification System for Numerous Scientific Applications
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- Akhras, Michael (författare)
- KTH,Skolan för bioteknologi (BIO)
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- Unemo, Magnus (författare)
- Örebro Univ Hosp, Dept Clin Microbiol
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- Thiyagarajan, Sreedevi (författare)
- Stanford Univ, Stanford Genome Technol Ctr
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- Nyrén, Pål (författare)
- KTH,Skolan för bioteknologi (BIO)
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- Davis, Ronald W. (författare)
- Stanford Univ, Stanford Genome Technol Ctr
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- Fire, Andrew Z. (författare)
- Stanford Univ
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- Pourmand, Nader (författare)
- Univ Calif Santa Cruz
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(creator_code:org_t)
- 2007-09-19
- 2007
- Engelska.
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 2:9, s. e915-
- Relaterad länk:
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system. As a proof-of-concept, this novel method, which employs a Connector Inversion Probe (CIPer), was tested as a genetic tool for pathogen diagnosis, typing, and antibiotic resistance screening with two distinct systems: i) a conserved sequence primer system for genotyping Human Papillomavirus (HPV), a cancer-associated viral agent and ii) screening for antibiotic resistance mutations in the bacterial pathogen Neisseria gonorrhoeae. We also discuss future applications and advances of the CIPer technology such as integration with digital amplification and next-generation sequencing methods. Furthermore, we introduce the concept of two-dimension informational barcodes, i. e. "multiplex multiplexing padlocks'' (MMPs). For the readers' convenience, we also provide an on-line tutorial with user-interface software application CIP creator 1.0.1, for custom probe generation from virtually any new or established primer-pairs.
Nyckelord
- DNA; DNA Probes; Drug Resistance
- Microbial; Mutation
- NATURAL SCIENCES
- NATURVETENSKAP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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