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Toward a Pinus pina...
Toward a Pinus pinaster bacterial artificial chromosome library
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- Bautista, Rocío (författare)
- University of Malaga
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- Villalobos, David, P. (författare)
- University of Malaga
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- Diaz-Moreno, Sara M (författare)
- University of Malaga
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- Cantón, Francisco, R. (författare)
- University of Malaga
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- Cánovas, Francisco, M. (författare)
- University of Malaga
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- Gonzalo Claros, M. (författare)
- University of Malaga
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(creator_code:org_t)
- Springer Science and Business Media LLC, 2007
- 2007
- Engelska.
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Ingår i: Annals of Forest Science. - : Springer Science and Business Media LLC. - 1286-4560 .- 1297-966X. ; 64:8, s. 855-864
- Relaterad länk:
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http://dx.doi.org/10...
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https://www.afs-jour...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Conifers are of great economic and ecological importance, but little is known concerning their genomic organization. This study is an attempt to obtain high-quality high-molecular-weight DNA from Pinus pinaster cotyledons and the construction of a pine BAC library. The preparation incorporates modifications like low centrifugation speeds, increase of EDTA concentration for plug maintenance, use of DNase inhibitors to reduce DNA degradation, use of polyvinylpyrrolidone and ascorbate to avoid secondary metabolites, and a brief electrophoresis of the plugs prior to their use. A total of 72 192 clones with an average insert size of 107 kb, which represents an equivalent of 11X pine haploid genomes, were obtained. The proportions of clones lacking inserts or containing chloroplast DNA are both approximately 1.6%. The library was screened with cDNA probes for seven genes, and two clones containing Fd-GOGAT sequences were found, one of them seemingly functional. Ongoing projects aimed at constructing a pinebacterial artificial chromosome library may benefit from the methods described here.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Skogsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Forest Science (hsv//eng)
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
Nyckelord
- BAC
- Glutamate synthase
- High molecular weight DNA
- Library
- Pine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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