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Development of a fe...
Development of a fed-batch process for the production of a recombinant protein X in CHO-GS system : Case study from the cell to reactor process ready for pilot scale cultivation
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- Chotteau, Veronique, 1963- (författare)
- KTH,Bioprocessteknik,Cell Technology Group
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- Jiang, Yun (författare)
- Biovitrum/SOBI, Sweden
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- westin, Jeanette (författare)
- Biovitrum/SOBI, Sweden
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- Dahlenborg, K (författare)
- Biovitrum/SOBI, Sweden
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- Sjöblom-Hallén, A (författare)
- Biovitrum/SOBI, Sweden
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- Svensson, Erik (författare)
- Biovitrum/SOBI, Sweden
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- Öberg, Mikael (författare)
- Biovitrum/SOBI, Sweden
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(creator_code:org_t)
- 2010-06-02
- 2010
- Engelska.
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Ingår i: Cells and Culture. - Dordrecht : Springer Science+Business Media B.V.. - 9789048134182 ; , s. 723-725
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A new cell line was created using CHO-GS system. The most promising clones were adapted to different base cultivation media leading to the selection of one medium. The fed-batch process development was performed in spinner, shake flask and bioreactor scale. It included the selection of a feed medium, the choice of the feed strategy and the optimisation of the glucose feeding. The process was then simplified by using a single feed including the feed medium and the glucose feed. Finally up-scaling parameters like aeration and CO2 stripping were studied in 3 L and 15 L bioreactors in preparation for pilot scale operation. This process proved to be robust, reproducible and suitable for large and commercial scale operation.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Läkemedelsbioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Pharmaceutical Biotechnology (hsv//eng)
Nyckelord
- CHO·GS·Fed-batch·Feed·Up-scale·Bioreactors
- SRA - Molecular Bioscience
- SRA - Molekylär biovetenskap
Publikations- och innehållstyp
- vet (ämneskategori)
- kon (ämneskategori)
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