Photosensitizer encapsulated organically modified silica nanoparticles for direct two-photon photodynamic therapy and In Vivo functional imaging

• Nanoparticle-assisted two-photon imaging and near infrared (NIR) imaging are two important technologies in biophotonics research. In the present paper, organically modified silica (ORMOSIL) nanoparticles encapsulated with either PpIX (protoporphyrin IX) photosensitizers or IR-820 NIR fluorophores were synthesized and optically characterized. Using the former ORMOSIL nanoparticles, we showed: (i) direct excitation of the fluorescence of PpIX through its efficient two-photon absorption in the intracellular environment of tumor cells, and (ii) cytotoxicity towards tumor cells by PpIX under two-photon irradiation. The latter ORMOSIL nanoparticles can be used as efficient NIR fluorescent contrast agents for various types . in vivo animal imaging. We applied IR-820 doped ORMOSIL nanoparticles in . in vivo brain imaging of mice. We also demonstrated the applications of them to sentinel lymph node (SLN) mapping of mice. Finally, we showed that the nanoprobes could target the subcutaneously xenografted tumor of a mouse for long time observations. ORMOSIL nanoparticles have great potentials for disease diagnosis and clinical therapies.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Elektroteknik och elektronik -- Annan elektroteknik och elektronik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Electrical Engineering, Electronic Engineering, Information Engineering -- Other Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)

Nyckelord

Fluorescence

In vivo imaging

NIR

ORMOSIL nanoparticles

Photodynamic therapy

Two-photon

Bio photonics

Brain imaging

Clinical therapy

Direct excitation

Disease diagnosis

Fluorescent contrast agents

Functional imaging

In-vivo

In-vivo animal imaging

In-Vivo imaging

Near-infrared imaging

Organically modified silica

ORMOSIL

Protoporphyrin IX

Sentinel lymph nodes

Tumor cells

Two photon

Two photon imaging

Two-photon absorptions

Two-photon photodynamic therapies

Diagnosis

Disease control

Infrared devices

Mammals

Nanoparticles

Photonics

Photons

Photosensitizers

Silica

Tumors

Two photon processes

Synthesis (chemical)

contrast medium

macrogol

nanoparticle

protoporphyrin

silicon dioxide

absorption

animal experiment

animal tissue

article

cytotoxicity

encapsulation

human

human cell

irradiation

light absorption

male

mouse

nanoprobe

neuroimaging

nonhuman

priority journal

sentinel lymph node

synthesis

tumor cell

xenograft

Animalia

Mus

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)