Sökning: id:"swepub:oai:DiVA.org:liu-100768" >
Probiotics modulate...
Probiotics modulate mast cell degranulation and reduce stress-induced barrier dysfunction in vitro
-
- Carlsson, Anders H. (författare)
- Linköpings universitet,Avdelningen för kliniska vetenskaper,Hälsouniversitetet
-
- Lutgendorff, Femke (författare)
- Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Gastrointestinal Research Unit, Department of Surgery, University Medical Center, Utrecht, The Netherlands
-
- Akkermans, Louis M.A. (författare)
- Gastrointestinal Research Unit, Department of Surgery, University Medical Center, Utrecht, The Netherlands.
-
visa fler...
-
- McKay, Derek M (författare)
- Gastrointestinal Research Group, Department of Physiology & Pharmacology, The Calvin, Phoebe and Joan Snyder Institute of Infection, Inflammation and Immunology, University of Calgary, Calgary, Alberta, Canada
-
- Söderholm, Johan D. (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Avdelningen för kliniska vetenskaper,Hälsouniversitetet,Kirurgiska kliniken US
-
visa färre...
-
(creator_code:org_t)
- 2013
- Engelska.
- Relaterad länk:
-
https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- BACKGROUND: Stress has well-established deleterious effects on intestinal barrier function and stressful life events are known to contribute to the development and perpetuation of inflammatory bowel diseases. Mast cells play a pivotal role in pathogenesis of stressinduced barrier dysfunction due to the release of barrier-disruptive content. Conversely, they also have recently been suggested to contribute to barrier protective properties of probiotics, through the release of 15d-PGJ2 and enhanced epithelial PPAR-γ activity. However, mechanisms remain to be elucidated.AIM: To study if probiotics can modulate mast cell mediator release, resulting in amelioration of stress-induced barrier dysfunction in vitro.METHODS: Confluent monolayers of the human colon-derived T84 epithelial cell line were co-cultured with rat basophilic leukemia (RBL)-2H3 mast cells and pretreated with probiotics (125x104 CFU/ml, 1hr) before addition of 100nM CRF to activate mast cells. Release of beta hexosaminidase, TNF-α and 15d-PGJ2 from mast cells was determined. Transepithelial resistance (TER), and permeability to microspheres (0.2μm) were measured over a 24h period. To determine dependence of PPAR-γ, monolayers were incubated with the specific PPAR-γ antagonist T0070907 before treatment with probiotics.RESULTS: CRF-induced activation of mast cells resulted in decreased TERs and increased permeability to microspheres. Both pretreatment with probiotics and filter-sterilized probiotic supernatant resulted in lower levels of mast cell-released beta hexosaminidase and TNF-α, and increased 15d-PGJ2. Furthermore, probiotics ameliorated epithelial barrier dysfunction in monolayers exposed to CRF-activated mast cells. However, when T84 monolayers were exposed to conditioned medium of CRF-activated mast cells or were incubated with T0070907, probiotics showed little or no effect.CONCLUSIONS: Probiotics modulate mast cell mediator release to a more barrier protective profile, resulting in amelioration of stress-induced epithelial barrier dysfunction, which is putatively mediated by a PPAR-γ dependent pathway.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)