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Identification of s...
Identification of sperm morphometric subpopulations in two different portions of the boar ejaculate and its relation to postthaw quality
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- Pena, FJ (författare)
- University of Extremadura, Cáceres, Spain
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- Saravia, F (författare)
- SLU, Sweden;
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- Garcia-Herreros, M (författare)
- University of Extremadura, Cáceres, Spain
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- Nunez-Martinez, I (författare)
- University of Extremadura, Cáceres, Spain
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- Tapia, JA (författare)
- University of Extremadura, Cáceres, Spain
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- Johannisson, A (författare)
- SLU, Sweden;
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- Wallgren, M (författare)
- SLU, Sweden;
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- Rodriguez-Martinez, Heriberto (författare)
- SLU, Sweden;
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(creator_code:org_t)
- American Society of Andrology, 2005
- 2005
- Engelska.
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Ingår i: Journal of Andrology. - : American Society of Andrology. - 0196-3635 .- 1939-4640. ; 26:6, s. 716-723
- Relaterad länk:
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https://onlinelibrar...
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https://urn.kb.se/re...
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https://doi.org/10.2...
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Abstract
Ämnesord
Stäng
- A statistical approach using sequentially principal component analysis (PCA), clustering, and discriminant analyses was. developed to identify sperm morphometric subpopulations in well-defined portions of the fresh boar ejaculate. Semen was obtained as 2 portions (the first 10 mL of the sperm-rich fraction and the rest of. the ejaculate, respectively) and frozen using a conventional protocol. Before freezing, an aliquot was Used for computer-assisted. sperm morphometry analysis (ASMA). Postthaw quality was evaluated using computer-assisted sperm analysis (CASA), and an annexin-V/PI assay evaluated sperm membranes. The PCA revealed that 3 variables represented more than 78% of the cumulative variance in sperm subpopulations. The clustering and discriminant analyses, based on 5780 individual spermatozoa, revealed the existence of 4 sperm subpopulations. The relative percentage of these subpopulations Varied between boar and ejaculate portions. Linear regression models based on measured morphometric characteristics could account for up to 36% of the percentage of intact sperm membranes postthaw. The ASMA protocol used in our study was useful to detect subtle morphometric differences between spermatozoa, and the combination of this analysis with a multivariate statistical procedure gave hew information on the biological characteristics of boar ejaculates that is not given by conventional sperm analysis.
Nyckelord
- ASMA; sperm subpopulations; cryopreservation; cluster analysis
- TECHNOLOGY
- TEKNIKVETENSKAP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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